Browsing by Author "Das, Anamika"

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    Amino-functionalized Magnetic Iron Nanoparticles As a Carrier for Laccase Enzyme and Its Potential to Degrade Chlorpyrifos in Contaminated Soil: Fate and Kinetics
    (Springer Science and Business Media Deutschland GmbH, 2022-04-14T00:00:00) Das, Anamika; Yogalakshmi, Kn
    The present study utilizes the covalent-crosslinking method to immobilize the�laccase enzyme on magnetic iron nanoparticles�and further assess its potential in degrading�chlorpyrifos in pesticide spiked�soil. The soil texture analysis revealed the presence of clay, silt and sand accounting to�7.1%, 14.3%, and 78.6%, respectively. The degradation of chlorpyrifos in spiked soil�using laccase enzyme�immobilized�magnetic iron nanoparticles was measured for 60�days�in a batch process. The results revealed a gradual increase in degradation efficiency showing around�29.3%, 43.8%, 56.3%, and 67.3% at the 20th, 30th, 40th, and 50th day�of incubation. A maximum degradation of�83.6% was observed on the 60th day of incubation. Intermediate products 2,4-bis(1,1 dimethylethyl) phenol, 1,2 benzenedicarboxylic acid, bis(2-methyl propyl) ester�and�piperidine confirmed the degradation of chlorpyrifos in soil. The piperidine originated in the chromatogram between�20�40�days of incubation and diminished later. Chlorpyrifos degradation followed pseudo first-order kinetics with a R2 of 0.96. Further, the nanoparticles showed no negative�impact on soil bacterial population during the antibacterial assay. The study confirms the degradation of chlorpyrifos in contaminated soil using laccase-immobilized nanoparticles. � 2022, The Author(s), under exclusive licence to Springer Nature Switzerland AG.
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    Comparative Analysis of Laccase Immobilization on Magnetic Iron Nanoparticles using Two Activating Agents:EDAC and Cyanuric Chloride
    (Tamil Nadu Scientific Research Organization (TNSRO), 2016) Das, Anamika; Singh, Jatinder; Yogalakshmi, K. N.
    Surface modification improves the covalent bonding of enzymes onto the magnetic nanoparticles. The present study aims to evaluate the effect of surface activators (EDAC (1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide) and Cyanuric chloride) in immobilization of enzymes. Nanoparticles prepared by co-precipitation method ranged insize from 15-20 nm. The nanoparticles possessed crystalline property as confirmed by the XRD (X-ray powder diffraction) peaks. SEM-EDS (Scanning Electron Microscopy- Energy Dispersive X-ray Spectroscopy) analysis of EDAC and cyanuric acid activated nanoparticles showed atomic sulphur percent of 0.08% and 0.02%, respectively. It is concluded that EDACwas more successful in loading more enzymes than cyanuric acid. Bradford estimation of the unbound protein after first wash for ENP-EDAC and ENP-CC was 29.1 μg/mL and 132.1 μg/mL, respectively. EDAC is a potential surface modifier for enzyme immobilization process.
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    Degradation of CHLORPYRIFOS by Laccase immobilized magnetic Iron Nanoparticles
    (Central University of Punjab, 2018) Das, Anamika; K.N. Yogalakshmi
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    Effect of natrium fluoride on growth and physiology of oryza sativa L.
    (Central University of Punjab, 2012) Das, Anamika; Mittal, Sunil
    The present study was undertaken to investigate the effect of natrium fluoride on growth and physiology of Oryza sativa L. Two varieties of the O. sativa (rice) viz. Basmati 1121 and PR147 were selected for the study. PR147 is a local variety of the region. The effect was studied on growth (root length and shoot length), physiological parameters (chlorophyll content and percent respiration), macromolecules and their hydrolyzing enzymes (carbohydrate and protein content, ?-amylase and protease activity) and antioxidant enzymes (peroxidase and superoxide dismutase) under controlled conditions in the laboratory. Studies revealed that NaF (in different concentrations ranging from 16, 32 and 64 mg/l) affected the growth and physiology of both varieties as compared to control. When the seed of both varieties of rice were exposed to 64 mg/l NaF concentration, the seedling growth of test plants was highly affected as compared to control. Basmati 1121 variety was found to be more sensitive than PR147. In chlorophyll content, the percent reduction at 64 mg/l of NaF was 62% in Basmati 1121 and 30% in PR147. Similarly, reduction in percent cellular respiratory ability on exposure to 64 mg/l of NaF was 75% and 22% in Basmati 1121 and PR147 variety respectively. At 64 mg/l the carbohydrate content was increased 2.9 folds in Basmati 1121 and 1.35 folds in PR147. However the protein content was variably affected as it was increased 2 folds in PR147 and decreased to nearly 55% in Basmati 1121. The macromolecular contents were affected as the activity of their hydrolyzing enzymes was also affected. The increase in oxidative stress due to high concentrations of fluoride exposure has lead to an increase in antioxidant enzyme activity of both peroxidase and superoxide dismutase enzymes. The study concludes that high content of fluoride affects the growth and physiology of O. sativa L. by inducing oxidative stress.