Comparative Analysis of Laccase Immobilization on Magnetic Iron Nanoparticles using Two Activating Agents:EDAC and Cyanuric Chloride
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Date
2016
Journal Title
Journal ISSN
Volume Title
Publisher
Tamil Nadu Scientific Research Organization (TNSRO)
Abstract
Surface modification improves the covalent bonding of enzymes onto the magnetic nanoparticles. The present study aims to evaluate the effect of surface activators (EDAC (1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide) and Cyanuric chloride) in immobilization of enzymes. Nanoparticles prepared by co-precipitation method ranged insize from 15-20 nm. The nanoparticles possessed crystalline property as
confirmed by the XRD (X-ray powder diffraction) peaks. SEM-EDS (Scanning Electron Microscopy- Energy Dispersive X-ray Spectroscopy) analysis of EDAC and cyanuric acid activated nanoparticles showed atomic sulphur percent of 0.08% and 0.02%, respectively. It is concluded that EDACwas more successful in loading more enzymes than cyanuric acid. Bradford estimation of the unbound protein after first wash for ENP-EDAC and ENP-CC was 29.1 μg/mL and 132.1 μg/mL, respectively. EDAC is a potential surface modifier for enzyme immobilization process.
Description
Keywords
Immobilization, EDAC, Cyanuric Chloride (CC), Enzyme, Nanoparticles, Laccase
Citation
Anamika Das, Jatinder Randhawa and Yogalakshmi. K.N. (2016), “Comparative analysis of Laccase immobilization on magnetic iron nanoparticles using two activating agents: EDAC and Cyanuric Chloride”, Indian Journal of Natural Sciences, Vol 6, Issue 35 Pp 10608-10615.