Comparative Analysis of Laccase Immobilization on Magnetic Iron Nanoparticles using Two Activating Agents:EDAC and Cyanuric Chloride

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dc.contributor.authorDas, Anamika
dc.contributor.authorSingh, Jatinder
dc.contributor.authorYogalakshmi, K. N.
dc.date.accessioned2017-07-25T10:26:57Z
dc.date.accessioned2024-08-14T06:39:31Z
dc.date.available2017-07-25T10:26:57Z
dc.date.available2024-08-14T06:39:31Z
dc.date.issued2016
dc.description.abstractSurface modification improves the covalent bonding of enzymes onto the magnetic nanoparticles. The present study aims to evaluate the effect of surface activators (EDAC (1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide) and Cyanuric chloride) in immobilization of enzymes. Nanoparticles prepared by co-precipitation method ranged insize from 15-20 nm. The nanoparticles possessed crystalline property as confirmed by the XRD (X-ray powder diffraction) peaks. SEM-EDS (Scanning Electron Microscopy- Energy Dispersive X-ray Spectroscopy) analysis of EDAC and cyanuric acid activated nanoparticles showed atomic sulphur percent of 0.08% and 0.02%, respectively. It is concluded that EDACwas more successful in loading more enzymes than cyanuric acid. Bradford estimation of the unbound protein after first wash for ENP-EDAC and ENP-CC was 29.1 μg/mL and 132.1 μg/mL, respectively. EDAC is a potential surface modifier for enzyme immobilization process.en_US
dc.identifier.citationAnamika Das, Jatinder Randhawa and Yogalakshmi. K.N. (2016), “Comparative analysis of Laccase immobilization on magnetic iron nanoparticles using two activating agents: EDAC and Cyanuric Chloride”, Indian Journal of Natural Sciences, Vol 6, Issue 35 Pp 10608-10615.en_US
dc.identifier.issn0976 –0997
dc.identifier.urihttp://10.2.3.109/handle/32116/203
dc.language.isoen_USen_US
dc.publisherTamil Nadu Scientific Research Organization (TNSRO)en_US
dc.subjectImmobilizationen_US
dc.subjectEDACen_US
dc.subjectCyanuric Chloride (CC)en_US
dc.subjectEnzymeen_US
dc.subjectNanoparticlesen_US
dc.subjectLaccaseen_US
dc.titleComparative Analysis of Laccase Immobilization on Magnetic Iron Nanoparticles using Two Activating Agents:EDAC and Cyanuric Chlorideen_US
dc.typeArticleen_US

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