Browsing by Author "Mahapatra, Santosh Kumar"

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Ex vivo interaction study of NaYF4:Yb,Er nanophosphors with isolated mitochondria
(John Wiley and Sons Inc, 2021-04-08T00:00:00) Singh, Varun Pratap; Chawda, Nitya; Barkhade, Tejal; Mahapatra, Santosh Kumar; Banerjee, Indrani
Ex vivo interaction of NaYF4:Yb,Er nanophosphors with isolated mitochondria has been investigated. The nanophosphors were synthesized using the hydrothermal method. The synthesized NaYF4:Yb,Er nanophosphors were characterized for physicochemical properties. The NaYF4:Yb,Er nanophosphors showed successful upconversion with excitation wavelength lying in the near-infrared region. The effect of synthesized NaYF4:Yb,Er nanophosphors on mitochondria isolated from the chicken heart tissue was examined through ROS generation capacity, membrane fluidity, and complex II activity. The exposer of NaYF4:Yb,Er nanophosphors to isolated mitochondria inhibits ROS generation activity as compared to control. The mitochondria membrane fluidity of the lipid bilayer and complex-II activity of mitochondria was observed to be unaltered after the interaction with NaYF4:Yb,Er nanoparticles. The results confirm that synthesized NaYF4:Yb,Er nanoparticles can be used as a safe contrast agent. � 2021 International Union of Biochemistry and Molecular Biology, Inc.
Homology modeling and molecular docking study of biogenic Muga silk nanoparticles as putative drug-binding system
(John Wiley and Sons Inc, 2020-12-22T00:00:00) Asapur, Prithvi C.; Sahare, Purushottam D.; Mahapatra, Santosh Kumar; Banerjee, Indrani
The recent emergence of natural biopolymers as drug delivery vehicles is attributed to their biodegradability and less systemic toxicity. Muga silk nanoparticles were prepared using microwave radiolysis method and were characterized by Fourier transform infrared spectroscopy, circular dichroism, X-ray diffraction and transmission electron microscopy. To find the applicability in the drug delivery system of these nanoparticle and to know the binding site(s), a computational study was carried out. The structure of the Muga protein is predicted using homology modeling, which is further used for molecular docking. The in silico molecular docking between the Muga silk nanoparticles and three United States Food and Drug Administration-approved model drugs of doxorubicin, remdesivir and dexamethasone was performed. The binding capabilities and binding energy of the Muga silk proteins with these drugs are determined. The basic idea of the active site and the residues involved in the binding of the drugs/ligands is also studied. Doxorubicin showed the highest binding affinity of ?8.7�kcal/mol and that of the remdesivir and dexamethasone are found to be ?7.2 and ?7.9 kcal/mol, respectively. Such high binding affinity(ies) would help for slow drug release kinetics and the other two drugs can be loaded when the requirement is for sustained drug release. The data were also validated using the UV�vis. spectroscopy. � 2020 International Union of Biochemistry and Molecular Biology, Inc.
Iron content titanium dioxide nanoparticles as exogenous contrast agent for tissue imaging using swept-source optical coherence tomography
(American Institute of Physics Inc., 2021-01-08T00:00:00) Barkhade, Tejal; Indoliya, Abhishek; Poddar, Raju; Mahapatra, Santosh Kumar; Banerjee, Indrani
Ex vivo tissue imaging was performed by swept-source optical coherence tomography (SS-OCT) using titanium dioxide (TiO2) and Fe content TiO2 nanoparticles (NPs). The comparative effects of TiO2 and Fe content TiO2 NPs in terms of contrast enhancement, penetration, scattering, and accumulation in the chicken breast tissue have been monitored at different exposure times. Powder NP samples were synthesized using the sol-gel method, and characterization was carried out via transmission electron microscopy, x-ray photoelectron spectroscopy, and diffuse reflectance spectroscopy techniques. Fe incorporation in the TiO2 matrix reduces its toxic effect on tissue skin and produces a safe exogenous contrast agent, which is analyzed by SS-OCT. The scattering coefficients and contrast to noise ratio of the tissues with and without NPs were determined to study the imaging efficacy. The improvement in the coefficient was observed with an increase in the exposure time of NPs. Nano-TiO2 has shown the ability to penetrate within the tissue layer up to 780 ?m while Fe content TiO2 NPs samples showed the lowest rate of penetration up to 210 ?m after a 30 min time interval. � 2021 Author(s).
A Protein and Membrane Integrity Study of TiO2 Nanoparticles-Induced Mitochondrial Dysfunction and Prevention by Iron Incorporation
(Springer, 2021-03-31T00:00:00) Barkhade, Tejal; Mahapatra, Santosh Kumar; Banerjee, Indrani
The paper assessed the toxic effect of titanium dioxide (TiO2) nanoparticles (NPs) on isolated mitochondria and its dysfunction prevention after Iron (Fe) incorporation. TiO2 and Fe content TiO2 NPs were synthesized and characterized using XPS, PL spectroscopy, and TEM. The nanostructure interaction with isolated mitochondria was investigated using circular dichroism (CD) confocal microscopy, flow cytometry, atomic force microscopy (AFM), surface-enhanced Raman spectroscopy (SERS), and FT-IR spectroscopy via nonspecific pathway. Fe content TiO2 NPs helps to control the dissolution rate of parent nanomaterial of TiO2 on the mitochondrial membrane. Confocal micrographs and flow cytometry results confirmed that Rhodamine 123 dye intensity get increased after interaction with Fe content TiO2 NPs which states the integrity of the mitochondrial membrane. AFM results revealed that TiO2 induces the swelling of mitochondrial tubules and also impaired the mitochondrial structure, whereas Fe content TiO2 NPs interaction prevents the impairment of mitochondrial tubules. The denaturation of a membrane protein by TiO2 interactions was observed through CD Spectroscopy. Further, nano-bio-interface study was performed using SERS, through shifting and extinct of peaks affiliated to membrane proteins and lipids. However, Fe content TiO2-treated samples showed a significant increase in the membrane potential of mitochondria via flow cytometry results. Graphic Abstract: [Figure not available: see fulltext.] � 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Secondary structural analysis of non-mulberry silk fibroin nanoparticles synthesized by using microwave and acetone method
(Taylor and Francis Ltd., 2020-12-04T00:00:00) Asapur, Prithvi; Mahapatra, Santosh Kumar; Banerjee, Indrani
The 3D structure analysis for Muga silk protein was predicted through homology modeling and Ramachandran plot with the determination of quality index for Stereo/packing and 3D quality index. Silk Nanoparticles were synthesized using conventional desolvation and microwave-assisted radiolysis method from Muga silk fibroin. The secondary structure evolution due to two different synthesis methods was investigated using FTIR and Circular Dichroism (CD) spectroscopy. The microwave synthesized nanoparticles showed enhanced ?-sheet content than desolvation synthesized nanoparticles. The conformational changes of Muga silk fibroin protein to nanoparticle followed an ordered transition from random coil to ?-helix then from ?-helix to ?-turn and from ?-turn to ?-sheets where ?-helix and ?-turn are the intermediate forms before getting stabilized to the metastable ?-sheets structure. The thermodynamics involved in secondary structure evolution was studied from CD analysis and activation energy involved in the formation of the secondary structure was determined. Communicated by Ramaswamy H. Sarma. � 2020 Informa UK Limited, trading as Taylor & Francis Group.
Surface-engineered gadolinium oxide nanorods and nanocuboids for bioimaging
(University of Science and Technology Beijing, 2020-04-04T00:00:00) Chawda, Nitya Ramesh; Mahapatra, Santosh Kumar; Banerjee, Indrani
Abstract: Folic acid and D-gluconic acid-capped gadolinium oxide nanorods and nanocuboids were synthesized via co-precipitation method. Comparative study of relaxivity factor on the role of capping and morphology for enhancing contrast ability for T1 and T2 magnetic resonance imaging (MRI) was investigated. The obtained r2/r1 ratio for folic acid and D-gluconic acid-capped gadolinium oxide nanorods and nanocuboids was 1.5 and 1.3, respectively. The nanocrystals were characterized and presented with properties such as good dispersity and stability required for standard contrast agent used in MRI. The characterization and the analysis of capping agent for nanocrystals suggest the preferable use of carbohydrate moieties with higher number of hydroxyl functional group reacted with urea and hydrogen peroxide for desired morphology and anisotropic growth. Thermogravimetric�differential thermal analysis (TG�DTA) illustrated the amount of capping, transition temperature from Gd(OH)3 to GdOOH and crystallization temperature from GdOOH to Gd2O3. These nanocrystals would be significant for other biomedical applications such as drug delivery when equipped with well-functionalized drug molecules. Graphic abstract: Synergistic effects and mechanism of urea, hydrogen peroxide and capping agent for growth and morphology. [Figure not available: see fulltext.] � 2020, The Nonferrous Metals Society of China and Springer-Verlag GmbH Germany, part of Springer Nature.