Ex vivo interaction study of NaYF4:Yb,Er nanophosphors with isolated mitochondria
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Date
2021-04-08T00:00:00
Authors
Singh, Varun Pratap
Chawda, Nitya
Barkhade, Tejal
Mahapatra, Santosh Kumar
Banerjee, Indrani
Journal Title
Journal ISSN
Volume Title
Publisher
John Wiley and Sons Inc
Abstract
Ex vivo interaction of NaYF4:Yb,Er nanophosphors with isolated mitochondria has been investigated. The nanophosphors were synthesized using the hydrothermal method. The synthesized NaYF4:Yb,Er nanophosphors were characterized for physicochemical properties. The NaYF4:Yb,Er nanophosphors showed successful upconversion with excitation wavelength lying in the near-infrared region. The effect of synthesized NaYF4:Yb,Er nanophosphors on mitochondria isolated from the chicken heart tissue was examined through ROS generation capacity, membrane fluidity, and complex II activity. The exposer of NaYF4:Yb,Er nanophosphors to isolated mitochondria inhibits ROS generation activity as compared to control. The mitochondria membrane fluidity of the lipid bilayer and complex-II activity of mitochondria was observed to be unaltered after the interaction with NaYF4:Yb,Er nanoparticles. The results confirm that synthesized NaYF4:Yb,Er nanoparticles can be used as a safe contrast agent. � 2021 International Union of Biochemistry and Molecular Biology, Inc.
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Keywords
fluidity, mitochondria, organelles, reactive oxygen species (ROS) generation, upconversion nanoparticles