Browsing by Author "Reetu"

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    Current Approaches and Key Applications of Plant Metabolic Engineering
    (Springer, 2018) Bharti; Reetu; Kumar, Vinay
    The diversion of carbon flux toward biosynthesis of targeted products could be achieved by manipulation of targeted biosynthesis pathway in plants. This whole process consists of many steps in stepwise manners starting with the identification and isolation of targeted metabolites, elucidation of complete biosynthetic pathway for identification of point of intervention, discovery of corresponding potential metabolic genes, and overexpression of the selected genes in heterologous system and collectively production of the metabolites. The various biochemical processes including transcriptome, translatome, proteome, and reactome are being used to assist metabolic engineering by providing new insights into novel pathways or bottlenecks of existing pathways. Apart from all these, in-depth understanding of metabolic fluxes and feedback regulations is also mandatory for plant metabolic engineering. All these different current approaches are collectively considered for investigating the plant metabolic engineering to understand, reconstruct, analyze, and annotate the targeted pathways. The key applications of plant metabolic engineering have been compiled with a few important applications including improvement of nitrogen utilization in plant, development of highly nutritive food, and generation of biofuel production. In conclusion, the plant metabolic engineering could provide comprehensive evaluation of manipulation of biosynthetic pathways for numerous applications. This compiled information could act as a resource for crop breeding and biotechnology purposes.
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    To Study Callus Induction from Explants of Nicotiana tabacum
    (Central University of Punjab, 2018) Reetu; Kumar,Vinay
    Due to a great importance of callus most of the work has been carried out on regeneration aspect of callus including somatic embryogenesis, which is very important aspect of plant tissue culture and crop improvement. A large number of plants have been regenerated in plant tissue facility using various explants in phytohormone supplemented MS media. In present proposal, three selected phytohormone (NAA, BAP and 2,4-D) in different concentration combinations have been used for callus induction using leaf and seedling as explants in tobacco. It was found that best combination for induction of callus is BAP and NAA as compared to BAP and 2,4-D. Color, texture and size of callus were assessed after 25 days of culture. Rate of growth was found more in the culture media containing BAP and NAA combination. It was observed that color of callus was greenish in culture media having BAP and NAA and yellowish brown in the culture medium containing BAP and 2,4-D. In conclusion, this work help to establish that both seedling and leaf explants can be used for callus formation with appropriate supply of phytohormones.