To Study Callus Induction from Explants of Nicotiana tabacum

Abstract

Due to a great importance of callus most of the work has been carried out on regeneration aspect of callus including somatic embryogenesis, which is very important aspect of plant tissue culture and crop improvement. A large number of plants have been regenerated in plant tissue facility using various explants in phytohormone supplemented MS media. In present proposal, three selected phytohormone (NAA, BAP and 2,4-D) in different concentration combinations have been used for callus induction using leaf and seedling as explants in tobacco. It was found that best combination for induction of callus is BAP and NAA as compared to BAP and 2,4-D. Color, texture and size of callus were assessed after 25 days of culture. Rate of growth was found more in the culture media containing BAP and NAA combination. It was observed that color of callus was greenish in culture media having BAP and NAA and yellowish brown in the culture medium containing BAP and 2,4-D. In conclusion, this work help to establish that both seedling and leaf explants can be used for callus formation with appropriate supply of phytohormones.