To Study Callus Induction from Explants of Nicotiana tabacum

dc.contributor.authorReetu
dc.contributor.supervisorKumar,Vinay
dc.date.accessioned2018-08-31T04:13:51Z
dc.date.accessioned2024-08-13T10:46:52Z
dc.date.available2018-08-31T04:13:51Z
dc.date.available2024-08-13T10:46:52Z
dc.date.issued2018
dc.description.abstractDue to a great importance of callus most of the work has been carried out on regeneration aspect of callus including somatic embryogenesis, which is very important aspect of plant tissue culture and crop improvement. A large number of plants have been regenerated in plant tissue facility using various explants in phytohormone supplemented MS media. In present proposal, three selected phytohormone (NAA, BAP and 2,4-D) in different concentration combinations have been used for callus induction using leaf and seedling as explants in tobacco. It was found that best combination for induction of callus is BAP and NAA as compared to BAP and 2,4-D. Color, texture and size of callus were assessed after 25 days of culture. Rate of growth was found more in the culture media containing BAP and NAA combination. It was observed that color of callus was greenish in culture media having BAP and NAA and yellowish brown in the culture medium containing BAP and 2,4-D. In conclusion, this work help to establish that both seedling and leaf explants can be used for callus formation with appropriate supply of phytohormones.en_US
dc.identifier.accessionnoT00768
dc.identifier.citationReetu (2018) To Study Callus Induction from Explants of Nicotiana tabacumen_US
dc.identifier.urihttps://kr.cup.edu.in/handle/32116/1959
dc.language.isoen_USen_US
dc.publisherCentral University of Punjaben_US
dc.subjectNicotiana tabacumen_US
dc.subjectCallus inductionen_US
dc.subjectPlant growth regulatorsen_US
dc.subject1- naphthaleneacetic aciden_US
dc.subject4-dichlorophenoxyacetic aciden_US
dc.subject6-benzylaminopurineen_US
dc.titleTo Study Callus Induction from Explants of Nicotiana tabacumen_US
dc.typeMaster Dissertationen_US

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