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Item 24-Epibrassinolide pre-treatment reduces alkaline-induced oxidative stress in red rice seedlings(Springer, 2019) Sharma, M; Mahajan, P; Singh, H.P; Batish, D.R; Kohli, R.K.Soil alkalinity caused by salts, such as sodium bicarbonate (NaHCO3), and the frequently associated waterlogging problems are pervasive in agriculture and have a deleterious impact on crop production. However, various plant growth regulators, including brassinosteroids, are considered to be important against different abiotic stresses experienced by plants due to drought, salinity, and heavy metal stress. We investigated the putative role of 24-epibrassinolide (EBL), an active brassinosteroid, on red rice plants experiencing alkaline stress. Seedlings were pre-treated with 0.01 μM EBL for 30 min and later, exposed to NaHCO3 (25 mM) and were sampled, 5 days after treatments. Results showed that the pre-treatment of seedlings with EBL under non-stress conditions could promote rice plant growth. Growth parameters including dry weight (DW), root and coleoptile lengths were reduced under alkaline stress, whereas EBL application reduced the level of inhibition, as compared with NaHCO3 treatment. Enhanced levels of malondialdehyde content, hydrogen peroxide, and superoxide radicals were significantly diminished by EBL pre-treatment. Moreover, pre-treatment of EBL to alkaline-treated rice seedlings largely stimulated the enzymatic activities of ascorbate peroxidase, catalase, and superoxide dismutase. Thus, the results suggest that pre-application of EBL significantly ameliorates alkaline stress in rice. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.Item Allelopathic Effect of Leaves of Invasive tree Broussonetia papyrifera against some crop plants(AdhyaBiosciences, 2016) Negi, Anjana; Batish, Daizy R.; Singh, Harminder Pal; Kohli, R.K.Allelopathic effect of aqueous extracts of leaves of Broussonetia papyrifera (L.) Vent. was studied on germination and seedling growth of Triticum aestivum L. and Oryza sativa L. under laboratory conditions. The seed germination, seedling length, seedling dry weight and total chlorophyll content were reduced with the increasing treatment of concentration (0.5, 1, 2 and 4%) of B. papyrifera leaf extract as compared to the control. The inhibitory effect was more pronounced on the root length than on coleoptile length. The extracts were rich in phenolic compounds, which are the ubiquitous allelochemicals and often implicated in allelopathy. The study concludes that B. papyrifera exhibits allelopathy through the release of phenolics from its leaves.Item Allelopathic effect of Pinus roxburghii on an understorey plant, Bidens pilosa(AdhyaBiosciences, 2016) Sharma, Neel Kanta; Batish, Daizy R.; Singh, Harminder Pal; Kohli, R.K.Allelopathic effect of Pinus roxburghii was studied on Bidens pilosa through laboratory and greenhouse experiments. The aqueous extracts of green needles, needle litter and bark were found to inhibit germination and initial growth of B. pilosa in laboratory bioassays. The inhibitory effect on germination root length, shoot length and biomass was also observed on amendment of powdered needle litter in soil in greenhouse pots. The inhibitory effect increased with increase in conc. of extracts or amount of litter amended. The amended soil was found to be rich in phenolics, the known potent allelochemics. The study indicates that such interactions may also be operational in forests of P. roxburghii.Item Allelopathic potential of essential oil of wild marigold (Tagetes minuta L) against some invasive weeds(Open Academic Journals Index, 2015) Arora,Komal; Batish, Daizy R.; Singh,Harminder Pal; Kohli, R.K.Tagetes minuta is an aromatic plant native to Tropical America. It exhibits wide range of biological activity against insects, nematodes, microbes including medicinal properties. It also creates nuisance for agricultural land. This may be attributed to its allelopathic properties. Therefore, the present study investigated the allelopathic potential of volatile oil of T. minuta on other invasive weeds - Chenopodium murale L., Phalaris minor Retz. and Amaranthus viridis L. It was observed that the volatile oil of T. minuta significantly reduced the germination, growth, chlorophyll content and respiratory ability of recipient weeds in a dose dependent manner. Mitotic studies revealed a complete arrest of mitotic activity in cells of treated root tips of Allium cepa with various aberrations like distorted, trinucleolated and binucleated cells. Thus, it can be concluded that the volatile oil of T. minuta shows allelopathic potential on other plants and this property could be further explored for weed management.Item Allelopathic potential of needle litter extracts of Pinus roxburghii on germination and early growth of Zea mays and Echinochloa crus-galli(Indian Journal of Applied Research (IJAR), 2016) Sharma, Neel Kanta; Batish, Daizy R.; Singh, Harminder Pal; Kohli, R.K.The needle litter of Pinus roxburghii, a common conifer of Himalayan region was investigated for allelopathic potential against crop plant Zea mays and weed Echinochloa crus-galli. The aqueous extracts from litter enhanced the growth of Z. mays slightly at lower concentrations but showed inhibitory effect at higher concentrations. However, the effect on E. crus- galli was inhibitory at all the concentrations. Overall, the effect on the weed species was more pronounced as compared to the crop species. The findings suggest that the litter of P. roxburghii possesses allelopathic potential and can serve as an important bioresource for management of weeds by utilizing the principles of allelopathy. However, further detailed field studies are needed.Item Allelopathic Potential of the Essential Oil of Wild Marigold (Tagetes minuta L.) Against Some Invasive Weeds(The Science Publisher, 2015) Arora, Komal; Batish, Daizy R.; Singh, Harminder Pal; Kohli, R.K.Tagetes minuta is an aromatic plant native to Tropical America. It exhibits wide range of biological activity against insects, nematodes, microbes including medicinal properties. It also creates nuisance for agricultural land. This may be attributed to its allelopathic properties. Therefore, the present study investigated the allelopathic potential of volatile oil of T. minuta on other invasive weeds - Chenopodium murale L., Phalaris minor Retz. and Amaranthus viridis L. It was observed that the volatile oil of T. minuta significantly reduced the germination, growth, chlorophyll content and respiratory ability of recipient weeds in a dose dependent manner. Mitotic studies revealed a complete arrest of mitotic activity in cells of treated root tips of Allium cepa with various aberrations like distorted, trinucleolated and binucleated cells. Thus, it can be concluded that the volatile oil of T. minuta shows allelopathic potential on other plants and this property could be further explored for weed management.Item Alterations in photosynthetic pigments, protein, and carbohydrate metabolism in a wild plant Coronopus didymus L. (Brassicaceae) under lead stress(Polish Academy of Sciences, 2017) Sidhu, G.P.S.; Singh, H.P.; Batish, Daizy R.; Kohli, R.K.Coronopus didymus has been emerged as a promising wild, unpalatable plant species to alleviate lead (Pb) from the contaminated soils. This work investigated the hypothesis regarding various metabolic adaptations of C. didymus under lead (Pb) stress. In pot experiments, we assessed the effect of Pb at varied concentrations (500?2900?mg?kg?1) on growth, photosynthetic pigments, alteration of macromolecular (protein and carbohydrate) content, and activities of enzymes like protease, ?-and ?-amylase, peroxidase (POX), and polyphenol oxidase (PPO) in C. didymus for 6?weeks. Results revealed that Pb exposure enhanced the growth, protein, and carbohydrate level, but decreased the leaf pigment concentration and activities of hydrolytic enzymes. The activities of POX and PPO in roots increased progressively by ~337 and 675%, respectively, over the control, at 2900?mg?kg?1 Pb treatment. Likewise, contemporaneous findings were noticed in shoots of C. didymus, strongly indicating its inherent potential to cope Pb-induced stress. Furthermore, the altered plant biochemical status and upregulated metabolic activities of POX and PPO indulged in polyphenol peroxidation elucidate their role in allocating protection and conferring resistance against Pb instigated stress. The current work suggests that stress induced by Pb in C. didymus stimulated the POX and PPO activities which impart a decisive role in detoxification of peaked Pb levels, perhaps, by forming physical barrier or lignifications. ? 2017, Franciszek G?rski Institute of Plant Physiology, Polish Academy of Sciences, KrakItem Analysis of Subsurface Soil Radon with the Environmental Parameters and Its Relation with Seismic Events(Springer, 2023-06-26T00:00:00) Jaishi, Hari Prasad; Singh, Sanjay; Tiwari, Raghavendra Prasad; Tiwari, Ramesh ChandraThis study reports continuous measurements of subsurface soil radon as well as environmental parameters for a period of three years. The survey was carried out along the active fault area in the Indo-Myanmar subduction zone in the north-eastern part which lies in the highest seismic zone of India. The wavelet-based decomposition of the environmental parameters was done using discrete wavelet transformation technique. The denoised environmental parameters by discrete wavelet transformation technique was fed as the inputs to the MLR (multiple linear regression) and MLP (multilayer perceptron) models. Residual radon was calculated and correlated with nearby seismic events. Many events of magnitude greater than or equal to 5 have occurred in the investigation area. It was possible to successfully correlate one event with the anomalous variation in soil radon. The correlated event was the only one with the shallow epicentral depth indicating that the investigated area has undergone a shallow rock fracturing due to the stress generated before the occurrence of the seismic event. � 2023, Geological Society of India, Bengaluru, India.Item Appraisal of immediate and late effects of mobile phone radiations at 2100 MHz on mitotic activity and DNA integrity in root meristems of Allium cepa(Springer, 2019) Chandel, S; Kaur, S; Issa, M; Singh, H.P; Batish, D.R; Kohli, R.K.The present study evaluated the potential of 2100 MHz radiofrequency radiations to act as cytotoxic and genotoxic agent. Fresh onion (Allium cepa L.) roots were exposed to electromagnetic field radiations (EMF-r) for different durations (1 h and 4 h) and evaluated for mitotic index (MI), phase index, chromosomal aberrations, and DNA damage. DNA damage was investigated with the help of the comet assay by assessing various parameters like % head DNA (HDNA), % tail DNA (TDNA), tail moment (TM), and olive tail moment (OTM). Effects of EMF-r exposure were also compared with that of methyl methanesulfonate (MMS; 90 μM), which acted as a positive control. The post-exposure effects of EMF-r after providing the test plants with an acclimatization period of 24 h were also evaluated. Compared to the control, a significant increase in the MI and aberration percentage was recorded upon 4 h of exposure. However, no specific trend of phase index in response to exposure was detected. EMF-r exposure incited DNA damage with a significant decrease in HDNA accompanied by an increase in TDNA upon exposure of 4 h. However, TM and OTM did not change significantly upon exposure as compared to that of control. Analysis of the post-exposure effects of EMF-r did not show any significant change/recovery. Our data, thus, suggest the potential cytotoxic and genotoxic nature of 2100 MHz EMF-r. Our study bears great significance in view of the swiftly emergent EMF-r in the surrounding environment and their potential for inciting aberrations at the chromosomal level, thus posing a genetic hazard. © 2019, Springer-Verlag GmbH Austria, part of Springer Nature.Item Appraisal of immediate and late effects of mobile phone radiations at 2100�MHz on mitotic activity and DNA integrity in root meristems of Allium cepa(Springer-Verlag Wien, 2019) Chandel S.; Kaur S.; Issa M.; Singh H.P.; Batish D.R.; Kohli R.K.The present study evaluated the potential of 2100�MHz radiofrequency radiations to act as cytotoxic and genotoxic agent. Fresh onion (Allium cepa L.) roots were exposed to electromagnetic field radiations (EMF-r) for different durations (1�h and 4�h) and evaluated for mitotic index (MI), phase index, chromosomal aberrations, and DNA damage. DNA damage was investigated with the help of the comet assay by assessing various parameters like % head DNA (HDNA), % tail DNA (TDNA), tail moment (TM), and olive tail moment (OTM). Effects of EMF-r exposure were also compared with that of methyl methanesulfonate (MMS; 90�?M), which acted as a positive control. The post-exposure effects of EMF-r after providing the test plants with an acclimatization period of 24�h were also evaluated. Compared to the control, a significant increase in the MI and aberration percentage was recorded upon 4�h of exposure. However, no specific trend of phase index in response to exposure was detected. EMF-r exposure incited DNA damage with a significant decrease in HDNA accompanied by an increase in TDNA upon exposure of 4�h. However, TM and OTM did not change significantly upon exposure as compared to that of control. Analysis of the post-exposure effects of EMF-r did not show any significant change/recovery. Our data, thus, suggest the potential cytotoxic and genotoxic nature of 2100�MHz EMF-r. Our study bears great significance in view of the swiftly emergent EMF-r in the surrounding environment and their potential for inciting aberrations at the chromosomal level, thus posing a genetic hazard. � 2019, Springer-Verlag GmbH Austria, part of Springer Nature.Item Appraisal of phytotoxic, cytotoxic and genotoxic potential of essential oil of a medicinal plant Vitex negundo(Elsevier B.V., 2020) Issa M.; Chandel S.; Pal Singh H.; Rani Batish D.; Kumar Kohli R.; Singh Yadav S.; Kumari A.The excessive use of synthetic herbicides in agriculture has steered the development of resistance in weeds along with the production of toxic effects on the environment. Essential oils (EOs) are emerging as an alternative to the synthetic herbicides or agrochemicals because of their effectiveness and easy degradability. A study was, therefore, planned to investigate the phytotoxic, cytotoxic and genotoxic potential of EO extracted from Vitex negundo L., a medicinally important plant. Chemical characterization using GC–MS revealed that V. negundo EO is rich in sesquiterpenes with ?-Caryophyllene (27.80 %) as the major compound. The phytotoxic effect of the EO (0.10–2.50 mg/mL) was investigated against two agricultural weeds: Avena fatua L. and Echinochloa crus-galli (L.) P. Beauv. Germination percentage and early seedling growth (coleoptile and root length) decreased significantly with an increase in EO concentration in both the test weeds. The cytotoxic potential of the oil was explored in Allium cepa L. by accessing mitotic index (MI) and chromosomal aberration percentage, whereas the effect on DNA integrity was evaluated as a percentage of head DNA (HDNA) and tail DNA (TDNA), tail moment (TM) and olive tail moment (OTM). EO treatment altered the cell cycle as evinced by a significant decrease in MI and an increase in aberration percentage at concentrations ? 0.05 and ? 0.025 mg/mL, respectively. In response to 0.10 mg/mL EO treatment, HDNA decreased by 9.37 %, whereas an increase of ?1.67, 4.70 and 1.80 fold was observed in TDNA, TM and OTM, respectively. The study concludes that V. negundo EO induced cytotoxic and genotoxic effects might be accountable for the phytotoxicity of EO against weeds, and thus holds a good potential for use under sustainable agricultural systems.Item Appraising the role of environment friendly chelants in alleviating lead by Coronopus didymus from Pb-contaminated soils(Elsevier Ltd, 2017) Sidhu, Gagan Preet Singh; Singh, Harminder Pal; Batish, Daizy R.; Kohli, R.K.In a screenhouse experiment, we investigated the role of two environment friendly chelants, Ammonium molybdate and EDDS for Pb mobilisation and its extraction by Coronopus didymus under completely randomized controlled conditions. Seedlings of C.?didymus were grown in pots having Pb-contaminated soil (1200 and 2200?mg?kg?1) for 6 weeks. Plants were harvested, 1 week after the addition of A. molybdate and EDDS. Results revealed that A. molybdate and EDDS enhanced the uptake and accumulation of Pb in roots and shoots of C.?didymus. At 2200?mg?kg?1 Pb level, compared to Pb-alone treatment, the maximal concentration of Pb was increased upto ?10% and ?19%, in roots whereas ?8% and ?18%, respectively, in shoots on addition of 2?mmol?kg?1 A. molybdate and EDDS. Additionally, Pb?+?EDDS treatments enhanced the plant biomass and triggered strong antioxidative response, more efficaciously than Pb?+?A. molybdate and Pb-alone treated plants. In this study, EDDS relative to A. molybdate was more efficient in mobilising and extracting Pb from soil. Although, EDDS followed by A. molybdate had good efficacy in mitigating Pb from contaminated soils but C.?didymus itself has the inherent affinity to tolerate and accumulate Pb from contaminated soils and hence in future, can be used either alone or with some other eco-friendly amendments for soil remediation purposes. ? 2017 Elsevier LtdItem A Bibliometric Assessment Of India's Library And Information Science Research Publications During 2011-2020(University of Idaho Library, 2021-06-09T00:00:00) Srivastava, Prachi; Sonkar, Sharad Kumar; Singh, RupinderThe aim of this paper is to conduct a bibliometric study to investigate the research output of library and information science in India. The SCOPUS database was selected to collect data from 2011 to 2020. Collected data was analysed on various parameters such as authorship pattern, year wise contribution of articles, most cited articles, top journals, top authors, document types, etc. The study found that in year 2019 Indian author contributed 471 research papers which 21.8% and highest in 10 years. Again in 2019, double authored article was highest in number with total of 252 articles. In periods of 10 years, about 2159 Indian articles were published with international collaboration. The growth of LIS research has been observed to bincreasing till 2019, but decrease in 2020 possibly due to the Covid pandemic. � 2021, Library Philosophy and Practice. All Rights Reserved.Item Bioaccumulation and physiological responses to lead (Pb) in Chenopodium murale L(Academic Press, 2018) Sidhu, G.P.S.; Bali A.S.; Bhardwaj R.; Singh H.P.; Batish D.R.; Kohli R.K.Identification and screening of wild, wasteland plants for the remediation of heavy metal contaminated soils is a crucial phytotechnological approach. In a pot experiment, Chenopodium murale was tested for the phytoextraction of lead (Pb) from the contaminated soils. The experiment was conducted for 8 weeks to appraise the effect of varied Pb regimes (300, 400, 500 mg kg?1) on the bioaccumulation and physiological responses of C. murale plants. At 500 mg kg?1 Pb regime, the concentration of Pb in roots and shoots reached a maximum of 2513 and 2301 mg kg?1 DW, respectively. The plants highlighted a very high tolerance with 100% survival towards Pb toxicity, although exhibited a slight decrease in biomass yield, chlorophyll content and protein levels. However, a dramatic mount in H2O2 content, MDA level and bio-activities of antioxidant enzymes (SOD, CAT and POD) was noticed. BCF and TF values were more than unity at all the Pb regimes. Results were scrutinized, accentuating the profit of raising C. murale in Pb-contaminated soils as this plant species can be a promising candidate for soil remediation and their restoration purposes.Item Biochemical Adaptations in Zea mays Roots to Short-Term Pb2+ Exposure: ROS Generation and Metabolism(Springer, 2015) Kaur, Gurpreet; Kaur, Shubhpreet; Singh,Harminder Pal; Batish, Daizy Rani; Kohli, R.K.; Rishi, ValbhaThe present study investigated the effect of lead (0, 16, 40 and 80 mg L?1 Pb2+) exposure for 3, 12 and 24 h on root biochemistry in hydroponically grown Zea mays (maize). Pb2+ exposure (80 mg L?1) enhanced malondialdehyde content (239 %�427 %), reactive carbonyl groups (425 %�512 %) and H2O2 (129 %�294 %) accumulation during 3�24 h of treatment, thereby indicating cellular peroxidation and oxidative damage. The quantitative estimations were in accordance with in situ detection of ROS generation (using 2?,7?-dichlorodihydrofluorescein diacetate dye) and H2O2 accumulation. Pb2+ treatment significantly reduced ascorbate and glutathione content during 3�24 h of exposure. On the contrary, levels of non-protein thiols were enhanced by 3�11.8 time over control in response to 16�80 mg L?1 Pb2+ treatment, after 24 h. A dose-dependent induction in ascorbate peroxidase and lipoxygenase enzyme activity was observed in Z. mays roots. The activities of ascorbate-recycling enzymes (dehydroascorbate reductase and monodehydroascorbate reductase) were significantly increased in relation to concentration and duration of Pb2+ treatment. The study concludes that Pb2+-exposure induces ROS-mediated oxidative damage during early period of exposure despite the upregulation of enzymes of ascorbate�glutathione cycle.Item Chemical Characterization and Phytotoxicity of Foliar Volatiles and Essential Oil of Callistemon viminalis(Har Krishan Bhalla and Sons, 2017) Bali, Aditi Shreeya; Batish, Daizy R.; Singh, Harminder Pal; Kaur, Shalinder; Kohli, R.K.We investigated the chemical composition and phytotoxicity of foliar volatiles (directly released from the macerated leaves) and essential oil extracted from the leaves of Callistemon viminalis against four weed species. Essential oil (EO) and foliar volatiles caused reduction in germination, seedling growth and dry matter accumulation in Bidens pilosa, Cassia occidentalis, Echinochloa crus-galli and Phalaris minor. Bidens pilosa was found to be the most sensitive towards foliar volatiles and EO, whereas C. occidentalis was the least sensitive. The chemical analyses of foliar volatiles and EO revealed the presence of 1,8-cineole and ?-pinene as the main monoterpenes. The study concludes that volatile components of C. viminalis possess phytotoxicity against weeds and thus may hold promise for the management of weeds under sustainable agriculture. ? 2017, Har Krishan Bhalla & Sons.Item Chemical characterization, phytotoxic, and cytotoxic activities of essential oil of Mentha longifolia(Springer, 2020) Singh, N; Singh, H.P; Batish, D.R; Kohli, R.K; Yadav, S.S.The present study assessed the phytotoxic and cytotoxic potential of the essential oil (EO) extracted from aboveground parts of Mentha longifolia (L.) Huds. Gas chromatography-mass spectrometry revealed 39 compounds constituting 99.67% of the EO. The EO was rich in monoterpenoids (mostly oxygenated monoterpenes), which accounted for 89.28% of the oil. The major components in EO were monoterpene ketones such as piperitone oxide (53.83%) and piperitenone oxide (11.52%), followed by thymol (5.80%), and (E)-caryophyllene (4.88%). The phytotoxic activities of EO were estimated against Cyperus rotundus, Echinochloa crus-galli, and Oryza sativa (rice) through pre- and post-emergence assays at concentrations ranging from 10 to 250 μg/ml and 0.5–5%, respectively. In pre-emergence assay, the phytotoxic effect of EO was most pronounced on C. rotundus, thereby significantly affecting percent germination, plantlet growth, and chlorophyll content. On the contrary, the impact was comparatively lesser on rice, with ~ 40% germination in response to 250 μg/ml of EO treatment. In the post-emergence assay, the spray treatment of EO caused a loss of chlorophyll and wilting in test plants, and subsequently affected the growth of plants, even leading to death in some cases. The cytotoxic activity of (at 2.5–50 μg/ml) was studied in meristem cells in onion (Allium cepa L.) root tips. EO exposure to the onion roots induced various chromosomal aberrations such as chromosomal bridges, c-mitosis, stickiness, vagrant chromosomes, etc., and negatively affected the mitotic index. At 50 μg/ml, EO treatment triggered the complete death of roots. The study concludes that M. longifolia EO has phytotoxic activities due to the mito-depressive effect, along with other physiological effects on target plants. Therefore, EO of M. longifolia could be developed into a novel bioherbicide for sustainable management of weeds in agricultural systems. - 2020, Springer-Verlag GmbH Germany, part of Springer Nature.Item Chemical profiling, cytotoxicity and phytotoxicity of foliar volatiles of Hyptis suaveolens(Academic Press, 2019) Sharma, A; Singh, H.P; Batish, D.R; Kohli, R.K.In the present study, the essential oil (EO) of Hyptis suaveolens has been explored for the first time for its phytotoxic and cytotoxic activities. The phytotoxic activity was assessed against rice (Oryza sativa) and its major troublesome weed, Echinochloa crus-galli, under laboratory and screenhouse conditions. GC-MS analysis revealed EO to be monoterpenoid (~ 79% monoterpenes) in nature with α-phellandrene (22.8%), α-pinene (10.1%) and limonene (8.5%) as the major chemical constituents. The laboratory bioassay showed a complete growth inhibitory effect of EO (≥ 2 mg mL −1 ) towards the germination and seedling growth of E. crus-galli. However, the inhibitory effect on rice was much less (~40% inhibition). EO caused visible injury, reduction in chlorophyll content, cell viability and ultimately led to complete wilting of E. crus-galli plants. In addition, EO altered the cell division in the meristematic cells of Allium cepa as depicted by ~63% decrease in mitotic index. EO exposure induced several aberrations at chromosomal (c-mitosis, anaphase bridges, chromosomal breakage, vagrant chromosomes, and sticky chromosomes) and cytological level (cytoplasm destruction, peripheral nuclei, and bi-nucleate cells). The present study concludes that H. suaveolens EO possesses phytotoxic activity due to its mito-depressive activity, and could serve as a natural herbicide under sustainable agricultural practices. © 2018 Elsevier Inc.Item Climate change and biodiversity: Analysis of the policy framework(IOS Press, 2014) Gupta, H.; Kohli, R.K.; Ahluwalia, A.S.; Pal, P.ASEAN countries are highly vulnerable to climate change and loss of biodiversity as a large proportion of their population is concentrated along coastlines, and heavily reliant on agriculture, natural resources and forestry for livelihoods. Seven of the ten countries have ratied both the UNFCCC and CBD, but they have been slow in taking up individual stands at CoP meetings. Three ASEAN countries are megabiodiverse. ASEAN countries were quick in framing and implementing domestic policies on conservation and climate change. One reason for this is that they preferred to go along with the G-77/China group during the negotiations. However, they have been more willing to take collective positions in CBD than in UNFCCC. ASEAN is pursuing a broad-based approach in taking voluntary mitigation and adaptation measuresItem Comparative account of allelopathic potential of essential oil of Tagetes minuta L. and its major component cis-β-Ocimene(AdhyaBiosciences, 2016) Arora, Komal; Batish, Daizy R.; Singh, Harminder Pal; Kohli, R.K.The study aims to explore the chemical composition of volatile essential oil of Tagetes minuta and comparison of its allelopathic potential with cis-β-ocimene, a major component of oil. T. minuta L. is an exotic aromatic plant found growing in northern plains and western Himalayas in India. The water distilled essential oil from aerial parts of T. minuta (at flowering stage) was analyzed by GC-MS. The analysis revealed 27 compounds representing 95.73% of the oil. cis-b-Ocimene (44.56%), dihydrotagetone (28.52%), limonene (3.99%) and tagetone (7.42%) were its major compounds. A comparison of allelopathic effect of cis-b-ocimene was made with T. minuta oil using Cassia occidentalis L., a common wasteland weed, as test plant. Laboratory study included growth studies in the form of germination, seedling length and dry weight of test plant under both treatments i.e. T. minuta oil and cis-b-ocimene. The inhibitory effect of ocimene was more significant as compared to oil. However, highest concentration (20µg/cc) of both treatments was found to be inhibitory with
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