Administration - Research Publications
Permanent URI for this collectionhttps://kr.cup.edu.in/handle/32116/2
Browse
2 results
Search Results
Item Chemical characterization, phytotoxic, and cytotoxic activities of essential oil of Mentha longifolia(Springer, 2020) Singh, N; Singh, H.P; Batish, D.R; Kohli, R.K; Yadav, S.S.The present study assessed the phytotoxic and cytotoxic potential of the essential oil (EO) extracted from aboveground parts of Mentha longifolia (L.) Huds. Gas chromatography-mass spectrometry revealed 39 compounds constituting 99.67% of the EO. The EO was rich in monoterpenoids (mostly oxygenated monoterpenes), which accounted for 89.28% of the oil. The major components in EO were monoterpene ketones such as piperitone oxide (53.83%) and piperitenone oxide (11.52%), followed by thymol (5.80%), and (E)-caryophyllene (4.88%). The phytotoxic activities of EO were estimated against Cyperus rotundus, Echinochloa crus-galli, and Oryza sativa (rice) through pre- and post-emergence assays at concentrations ranging from 10 to 250 μg/ml and 0.5–5%, respectively. In pre-emergence assay, the phytotoxic effect of EO was most pronounced on C. rotundus, thereby significantly affecting percent germination, plantlet growth, and chlorophyll content. On the contrary, the impact was comparatively lesser on rice, with ~ 40% germination in response to 250 μg/ml of EO treatment. In the post-emergence assay, the spray treatment of EO caused a loss of chlorophyll and wilting in test plants, and subsequently affected the growth of plants, even leading to death in some cases. The cytotoxic activity of (at 2.5–50 μg/ml) was studied in meristem cells in onion (Allium cepa L.) root tips. EO exposure to the onion roots induced various chromosomal aberrations such as chromosomal bridges, c-mitosis, stickiness, vagrant chromosomes, etc., and negatively affected the mitotic index. At 50 μg/ml, EO treatment triggered the complete death of roots. The study concludes that M. longifolia EO has phytotoxic activities due to the mito-depressive effect, along with other physiological effects on target plants. Therefore, EO of M. longifolia could be developed into a novel bioherbicide for sustainable management of weeds in agricultural systems. - 2020, Springer-Verlag GmbH Germany, part of Springer Nature.Item Chemical profiling, cytotoxicity and phytotoxicity of foliar volatiles of Hyptis suaveolens(Academic Press, 2019) Sharma, A; Singh, H.P; Batish, D.R; Kohli, R.K.In the present study, the essential oil (EO) of Hyptis suaveolens has been explored for the first time for its phytotoxic and cytotoxic activities. The phytotoxic activity was assessed against rice (Oryza sativa) and its major troublesome weed, Echinochloa crus-galli, under laboratory and screenhouse conditions. GC-MS analysis revealed EO to be monoterpenoid (~ 79% monoterpenes) in nature with α-phellandrene (22.8%), α-pinene (10.1%) and limonene (8.5%) as the major chemical constituents. The laboratory bioassay showed a complete growth inhibitory effect of EO (≥ 2 mg mL −1 ) towards the germination and seedling growth of E. crus-galli. However, the inhibitory effect on rice was much less (~40% inhibition). EO caused visible injury, reduction in chlorophyll content, cell viability and ultimately led to complete wilting of E. crus-galli plants. In addition, EO altered the cell division in the meristematic cells of Allium cepa as depicted by ~63% decrease in mitotic index. EO exposure induced several aberrations at chromosomal (c-mitosis, anaphase bridges, chromosomal breakage, vagrant chromosomes, and sticky chromosomes) and cytological level (cytoplasm destruction, peripheral nuclei, and bi-nucleate cells). The present study concludes that H. suaveolens EO possesses phytotoxic activity due to its mito-depressive activity, and could serve as a natural herbicide under sustainable agricultural practices. © 2018 Elsevier Inc.