Administration - Research Publications

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Author: search.filters.author.Singh,Harminder Pal

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    Biochemical Adaptations in Zea mays Roots to Short-Term Pb2+ Exposure: ROS Generation and Metabolism
    (Springer, 2015) Kaur, Gurpreet; Kaur, Shubhpreet; Singh,Harminder Pal; Batish, Daizy Rani; Kohli, R.K.; Rishi, Valbha
    The present study investigated the effect of lead (0, 16, 40 and 80 mg L?1 Pb2+) exposure for 3, 12 and 24 h on root biochemistry in hydroponically grown Zea mays (maize). Pb2+ exposure (80 mg L?1) enhanced malondialdehyde content (239 %�427 %), reactive carbonyl groups (425 %�512 %) and H2O2 (129 %�294 %) accumulation during 3�24 h of treatment, thereby indicating cellular peroxidation and oxidative damage. The quantitative estimations were in accordance with in situ detection of ROS generation (using 2?,7?-dichlorodihydrofluorescein diacetate dye) and H2O2 accumulation. Pb2+ treatment significantly reduced ascorbate and glutathione content during 3�24 h of exposure. On the contrary, levels of non-protein thiols were enhanced by 3�11.8 time over control in response to 16�80 mg L?1 Pb2+ treatment, after 24 h. A dose-dependent induction in ascorbate peroxidase and lipoxygenase enzyme activity was observed in Z. mays roots. The activities of ascorbate-recycling enzymes (dehydroascorbate reductase and monodehydroascorbate reductase) were significantly increased in relation to concentration and duration of Pb2+ treatment. The study concludes that Pb2+-exposure induces ROS-mediated oxidative damage during early period of exposure despite the upregulation of enzymes of ascorbate�glutathione cycle.
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    Allelopathic potential of essential oil of wild marigold (Tagetes minuta L) against some invasive weeds
    (Open Academic Journals Index, 2015) Arora,Komal; Batish, Daizy R.; Singh,Harminder Pal; Kohli, R.K.
    Tagetes minuta is an aromatic plant native to Tropical America. It exhibits wide range of biological activity against insects, nematodes, microbes including medicinal properties. It also creates nuisance for agricultural land. This may be attributed to its allelopathic properties. Therefore, the present study investigated the allelopathic potential of volatile oil of T. minuta on other invasive weeds - Chenopodium murale L., Phalaris minor Retz. and Amaranthus viridis L. It was observed that the volatile oil of T. minuta significantly reduced the germination, growth, chlorophyll content and respiratory ability of recipient weeds in a dose dependent manner. Mitotic studies revealed a complete arrest of mitotic activity in cells of treated root tips of Allium cepa with various aberrations like distorted, trinucleolated and binucleated cells. Thus, it can be concluded that the volatile oil of T. minuta shows allelopathic potential on other plants and this property could be further explored for weed management.