Administration - Research Publications
Permanent URI for this collectionhttps://kr.cup.edu.in/handle/32116/2
Browse
2 results
Search Results
Item Appraisal of phytotoxic, cytotoxic and genotoxic potential of essential oil of a medicinal plant Vitex negundo(Elsevier B.V., 2020) Issa M.; Chandel S.; Pal Singh H.; Rani Batish D.; Kumar Kohli R.; Singh Yadav S.; Kumari A.The excessive use of synthetic herbicides in agriculture has steered the development of resistance in weeds along with the production of toxic effects on the environment. Essential oils (EOs) are emerging as an alternative to the synthetic herbicides or agrochemicals because of their effectiveness and easy degradability. A study was, therefore, planned to investigate the phytotoxic, cytotoxic and genotoxic potential of EO extracted from Vitex negundo L., a medicinally important plant. Chemical characterization using GC–MS revealed that V. negundo EO is rich in sesquiterpenes with ?-Caryophyllene (27.80 %) as the major compound. The phytotoxic effect of the EO (0.10–2.50 mg/mL) was investigated against two agricultural weeds: Avena fatua L. and Echinochloa crus-galli (L.) P. Beauv. Germination percentage and early seedling growth (coleoptile and root length) decreased significantly with an increase in EO concentration in both the test weeds. The cytotoxic potential of the oil was explored in Allium cepa L. by accessing mitotic index (MI) and chromosomal aberration percentage, whereas the effect on DNA integrity was evaluated as a percentage of head DNA (HDNA) and tail DNA (TDNA), tail moment (TM) and olive tail moment (OTM). EO treatment altered the cell cycle as evinced by a significant decrease in MI and an increase in aberration percentage at concentrations ? 0.05 and ? 0.025 mg/mL, respectively. In response to 0.10 mg/mL EO treatment, HDNA decreased by 9.37 %, whereas an increase of ?1.67, 4.70 and 1.80 fold was observed in TDNA, TM and OTM, respectively. The study concludes that V. negundo EO induced cytotoxic and genotoxic effects might be accountable for the phytotoxicity of EO against weeds, and thus holds a good potential for use under sustainable agricultural systems.Item Appraisal of immediate and late effects of mobile phone radiations at 2100�MHz on mitotic activity and DNA integrity in root meristems of Allium cepa(Springer-Verlag Wien, 2019) Chandel S.; Kaur S.; Issa M.; Singh H.P.; Batish D.R.; Kohli R.K.The present study evaluated the potential of 2100�MHz radiofrequency radiations to act as cytotoxic and genotoxic agent. Fresh onion (Allium cepa L.) roots were exposed to electromagnetic field radiations (EMF-r) for different durations (1�h and 4�h) and evaluated for mitotic index (MI), phase index, chromosomal aberrations, and DNA damage. DNA damage was investigated with the help of the comet assay by assessing various parameters like % head DNA (HDNA), % tail DNA (TDNA), tail moment (TM), and olive tail moment (OTM). Effects of EMF-r exposure were also compared with that of methyl methanesulfonate (MMS; 90�?M), which acted as a positive control. The post-exposure effects of EMF-r after providing the test plants with an acclimatization period of 24�h were also evaluated. Compared to the control, a significant increase in the MI and aberration percentage was recorded upon 4�h of exposure. However, no specific trend of phase index in response to exposure was detected. EMF-r exposure incited DNA damage with a significant decrease in HDNA accompanied by an increase in TDNA upon exposure of 4�h. However, TM and OTM did not change significantly upon exposure as compared to that of control. Analysis of the post-exposure effects of EMF-r did not show any significant change/recovery. Our data, thus, suggest the potential cytotoxic and genotoxic nature of 2100�MHz EMF-r. Our study bears great significance in view of the swiftly emergent EMF-r in the surrounding environment and their potential for inciting aberrations at the chromosomal level, thus posing a genetic hazard. � 2019, Springer-Verlag GmbH Austria, part of Springer Nature.