Human Genetics And Molecular Medicine - Master Dissertation

Permanent URI for this collectionhttps://kr.cup.edu.in/handle/32116/104

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    Eeffect of Natural Compounds on Her2 Expression
    (Central University of Punjab, 2018) Uradanda, Praveen; Chander, Harish
    Breast cancer is a heterogeneous disease that is challenging to treat.we examine potential natural compounds which may help in treating her2 positive breast cancer. The main objectives of this study are to determine the mRNA level and protein levels on exposing MDAMB453 cell lines with Quercetin and PEITC at 40µm,44hrs.we have found in result analysis that her2 expression is downregulated on treatment with PEITC.where cell line treated with quercetin as no effect to downregulate her2 expression in MDAMB453.
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    Identification of Novel lncRNA in Breast Cancer Cell Line
    (Central University of Punjab, 2018) Sharma, Divya; Singh,Sandeep
    Long non-coding RNA(lncRNA) is RNA transcript having size of more 200 nucleotides and low number of exons are present due to which it expressed at lower level in specific tissues. Mainly the lncRNA is involved in gene regulations such as epigenetic regulation, chromatin remodeling and posttranscriptional regulations.The localization of lncrna is mainly in nucleus but there is considerable amount of lncrna present in cytoplasm. Lncrna can also target the mitochondrial genome. Identification of the targets of lncrna on mitochondrial genome helps to find the relationship between lncrna and mitochondrial functions which further helps in diagnosis and in therapy of the mitochondrial related diseases. One such lncRNA is MIR503HG, which act as a tumor suppressor in breast cancer cell line. Expression of lncRNA MIR503HG was analyzed in normoxic and hypoxic conditions upon the treatments of three different types of drugs on breast cancer cell line. The study of expression level of lncRNA MIR503HG in breast cancer cell line suggest a new cancer biomarker.