Botany - Research Publications

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Author: search.filters.author.Majeed, A.Has files: Yes

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    Transcriptome characterization and screening of molecular markers in ecologically important Himalayan species (Rhododendron arboreum)
    (Canadian Science Publishing, 2018) Choudhary, S.; Thakur, S.; Najar, R.A.; Majeed, A.; Singh, A.; Bhardwaj, P.; Crease, T.
    Rhododendron arboreum is an ecologically prominent species, which also lends commercial and medicinal benefits in the form of palatable juices and useful herbal drugs. Local abundance and survival of the species under a highly fluctuating climate make it an ideal model for genetic structure and functional analysis. However, a lack of genomic data has hampered additional research. In the present study, cDNA libraries from floral and foliar tissues of the species were sequenced to provide a foundation for understanding the functional aspects of the genome and to construct an enriched repository that will promote genomics studies in the genera. Illumina's platform facilitated the generation of ?100 million high-quality paired-end reads. De novo assembly, clustering, and filtering out of shorter transcripts predicted 113 167 non-redundant transcripts with an average length of 1164.6 bases. Of these, 71 961 transcripts were categorized based on functional annotations in the Gene Ontology database, whereby 5710 were grouped into 141 pathways and 23 746 encoded for different transcription factors. Transcriptome screening further identified 35 419 microsatellite regions, of which, 43 polymorphic loci were characterized on 30 genotypes. Seven hundred and nineteen transcripts had 811 high-quality single-nucleotide polymorphic variants with a minimum coverage of 10, a total score of 20, and SNP% of 50. ? 2018 Published by NRC Research Press.
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    Carrageenan based hydrogels and composites: A systematic investigation reporting their multidimentional essence
    (Nova Science Publishers, Inc., 2016) Ul Rehman, W.; Majeed, A.; Rani, P.; Saini, K.C.; Najar, R.A.; Mehra, R.; Singh, A.; Bast, Felix
    Carrageenans are red seaweed extracted sulfated linear polysaccharides which possess excellent gel forming properties. They are extensively used for their thickening, gel forming as well as stabilizing properties in food industries. Due to their good protein binding abilities, they are preferred in meat and dairy preservation. Structurally, they are polymers of alternately repeating disaccharide unit, ?-(133)-D-galactose and ?-(134)-3,6- anhydro-D- or L-galactose. Sulphation of the galactose gives them the distinguishing properties. Three isomeric forms of carrageenan with slightly different properties exist in nature. Their hydrogels are used in tissue engineering and controlled drug delivery while their composites find applications in wound dressings, protection of fish and meat from dehydration. In the field of medicine, carrageenans possess anticoagulant, antithrombotic, antiviral, and antitumor activities. Besides they also possess many industrial applications. ? 2016 Nova Science Publishers, Inc.
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    Gelatin: A comprehensive report covering its indispensable aspects
    (Nova Science Publishers, Inc., 2016) Ul Rehman, W.; Majeed, A.; Mehra, R.; Bhushan, S.; Rani, P.; Saini, K.C.; Bast, Felix
    Gelatin is a collagen derived product, obtained by incomplete hydrolysis of collagen procured from skin, bones and connective tissues of animals and exhibits flavourlessness, colourlessness and translucency. It is commonly utilised as a gelling agent and also as additive in food, drugs, cosmetics, paints, matches, photographic films and foam stabilizer. The overall amino acid composition and proportion of gelatin varies according to the source of raw material, however glycine, proline and hyrdoxyproline constitute almost 60% of the total amino acid residues while cysteine is absent. Besides being used in food industries, gelatin based composites and blends are used in pharmacy for manufacturing biocompatible gelatin scraps, tissue engineering films and controlled drug delivery systems. This chapter focuses on the physio-chemical properties of gelatin, its extraction, composites and blends. ? 2016 Nova Science Publishers, Inc.