Botany - Master Dissertation

Permanent URI for this collectionhttps://kr.cup.edu.in/handle/32116/29

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Evaluation of genotoxic potential of gropundwater from industrial and farmering sites on colon cancer cell lines
(Central University of Punjab, 2014) Kaur, Rimplejeet; Kumar, Sanjeev
Water is critical component on earth which is essential for life. There are number of reservoirs of water like surface water, groundwater etc. used for drinking, domestic as well as industrial purposes. Numerous studies have shown that water is contaminated by many pollutants like chemicals including pesticides, fertilizers, and heavy metals including arsenic, uranium, and exposure to this contaminated water may cause number of health problems including cancer. It has been found that in Punjab, specifically Malwa region's groundwater is highly contaminated by pesticides and heavy metals which may correlate to several health problems in this region. In this context, mechanisms of genotoxic potential activity of the groundwater, from thermal plant and villages Deon and Joganand, was estimated in the present study. The groundwater caused reactive oxygen species production, mitochondrial membrane depolarization, which ultimately caused cell death. Groundwater also affected the antioxidant system enzymes, so all these factors may be the cause of health problems in this region.
Status of endogenous antioxidants defense in preconditioned contrasting chickpea in precondioned contrasting chickpea (cicer arietinum) seedlingls
(Central University of Punjab, 2014) Jairath, Ankur; Kumar, Sanjeev
Plants experience various abiotic stresses of low or elevated temperature, drought and heavy metals. Chickpea (CicerarietinumL.) is one of the important crop belongs to the family Leguminosae and it is the second most important grain legume plant in the world after common bean. Chickpea has evolved as a spring crop in warmer climate of West Asia. Therefore, it is prone to chilling injury as in north India; chickpea is cultivated in winter season. To protect the plant from chilling injury, preconditioning the plants with mild drought stress might triggers the signalling pathways that will prepare the plants to combat lethal cold stress. This is determined by monitoring the various cell responses at different phases. Therefore antioxidative enzyme activity of the SOD, APX, GPOX, LOX, CAT enzymes was done. In our study, both the varieties, PDG3 and GPF2 showed equalvariation towards SOD, CAT, GPOX, and APX activity. Enhanced SOD, CAT & GPOX activity in both the varieties showed increased tolerance towards cold stress. Less LOX activity showed preconditioning decreased membrane damage in preconditioned seedlings. Thus, preconditioning with mild drought stress has enhanced tolerance capacity of the plant during the cold stress.