Department Of Botany

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Genetic diversity and population structure of Melia azedarach in North-Western Plains of India
(Springer Verlag, 2016) Thakur, Sapna; Choudhary, Shruti; Singh, Amandeep; Ahmad, Kamal; Sharma, Gagan; Majeed, Aasim; Bhardwaj, Pankaj
Key message: Genetic structure amongM. azedarachpopulations was detected and two subpopulations were present among them. A significant ?isolation by distance? was found inM. azedarachpopulation in North-Western Plains of India. Abstract: Melia azedarach is an important forest tree with pharmaceutical, insecticidal, pesticidal, and commercial significance. It is a good reforestation tree because of its fast growth and drought hardy nature. Genetic variation in a species allows itself to adapt, evolve and respond to environmental stress. It provides the basis for survival of a species and critically influences its evolutionary potential. Assessment of genetic diversity is necessary for improvement and conservation of a species. For this, microsatellite markers are of particular interest given the attributes like co-dominance, reproducibility, hyper variability and abundance throughout the genome. In the present study, we analyzed the genetic diversity and population structure of M. azedarach, an ecologically imperative species growing in the North-Western Plains of India. We developed 43 microsatellite markers, of which 20 were subsequently employed for analysis of diversity and population structure among 33 populations encompassing 318 genotypes representing North-Western Plains of India. A moderate level of diversity (Na?=?5.1, Ho?=?0.506, He?=?0.712, I?=?1.386) was assessed. The highest value of ?K estimated using STRUCTURE indicated 2 subpopulations (K?=?2). AMOVA exhibited 73?% variation within populations and 12?% variation was found among regions. Significant positive correlation between geographical and genetic distance was found (Rxy?=?0.365, P?=?0.010). The present study lays a foundation on a better understanding of genetic dynamics of the species and reveals its diversity and population structure in North-Western Plains of India. ? 2016, Springer-Verlag Berlin Heidelberg.
Population analysis of media azesarach L. by RAPD markers in the region of rajasthan
(Central University of Punjab, 2013) Ahmad, Kamal; Bhardwaj, Pankaj
Melia azedarach L is ecologically imperative species growing in Thar Desert. Biological effects such as antiviral, anthelminthic, antibacterial, etc. makes it important, yet a little is known about its genetic diversity and structure. In this study, we employed 30 RAPD primers for DNA profiling of 91 individuals representing 8 geographically isolated populations. A total of 98 bands were scored with an average of 3.2 bands per primer. The PIC ranged from 0.0681 to 0.5351 with an average of 0.4103. Nei's genetic diversity (h) and Shannon's information index (I) ranged from 0.1237 to 0.2375 and 0.1845 to 0.3500 respectively with average Nei'sgenetic diversity of 0.2570. The gene flow (Nm) 0.9295 and the genetic diversity of 0.3498 at species level demonstrated overall high level of genetic diversity. Cladistics analysis using DARwin and Bayesian cluster analysis using STRUCTURE placed 44 individuals into two main clusters or original genetic stocks (K = 2) which show little or no association with the geographic origin. The lack of clear assignment of individuals to geographical regions of sampling and consideration of different populations in the same genetic cluster suggests a recent common evolutionary history. M. azedarach is a good reforestation tree because of its fast growth and drought hardy nature. This study adds a foundation for more precise inference about the biogeography and management in the reforestation projects in the Thar Desert.