Department Of Botany

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Author: search.filters.author.Kumar, RashpalSubject: search.filters.subject.Chickpea

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    Drought priming induces chilling tolerance and improves reproductive functioning in chickpea (Cicer arietinum L.)
    (Springer Science and Business Media Deutschland GmbH, 2022-08-02T00:00:00) Saini, Rashmi; Das, Rangman; Adhikary, Arindam; Kumar, Rashpal; Singh, Inderjit; Nayyar, Harsh; Kumar, Sanjeev
    Key message: Priming alleviates membrane damage, chlorophyll degradation along with cryoprotectants accumulation during chilling stress that leads to improved reproductive functioning and increased seed yield. Abstract: Chilling temperatures below 15��C have severe implications on the reproductive growth and development of chickpea. The abnormal reproductive development and subsequent reproductive failure lead to substantial yield loss. We exposed five chickpea cultivars (PBG1, GPF2, PDG3, PDG4, and PBG5) to drought stress (Priming) during the vegetative stage and analyzed for chilling tolerance during the reproductive stage. These varieties were raised in the fields in two sets: one set of plants were subjected to drought stress at the vegetative stage for 30�days (priming) and the second set of plants were irrigated regularly (non-primed). The leaf samples were harvested at the flowering, podding, and seed filling stage and analyzed for membrane damage, water status, chlorophyll content, cellular respiration, and certain cryoprotective solutes. The reproductive development was analyzed by accessing pollen viability, in vivo and in vitro germination, pollen load, and in vivo pollen tube growth. Principal component analysis (PCA) revealed that priming improved membrane damage, chlorophyll b degradation, and accumulation of cryoprotectants in GPF2, PDG3, and PBG5 at the flowering stage (< 15��C). Pearson's correlation analysis showed a negative correlation with the accumulation of proline and carbohydrates with flower, pod, and seed abortion. Only, PBG5 responded best to priming while PBG1 was worst. In PBG5, priming resulted in reduced membrane damage and lipid peroxidation, improved water content, reduced chlorophyll degradation, and enhanced cryoprotective solutes accumulation, which led to increased reproductive functioning and finally improved seed yield and harvest index. Lastly, the priming response is variable and cultivar-specific but overall improve plant tolerance. � 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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    Understanding cross-tolerance mechanism and effect of drought priming on individual heat stress and combinatorial heat and drought stress in chickpea
    (Springer, 2022-03-15T00:00:00) Yadav, Renu; Juneja, Sumandeep; Kumar, Rashpal; Saini, Rashmi; Kumar, Sanjeev
    In northern India, chickpea experiences abrupt heat and drought stress during the late developmental stage and entails significant production loss. In the present study, the effect of heat stress and combined stress (heat and drought) was assessed in five varieties of chickpea along with the underlying mechanism of cross-tolerance by priming. Healthy seedlings (24�days old) were primed with mild drought stress and exposed to three different temperatures, i.e., 38��C, 35��C, and 32��C for 12, 24, and 36�h respectively, and also in combination with drought. The damage and tolerance were evaluated based on biochemical and physiological indicators. Results indicate that significant response was observed at 35��C as compared to 32 and 38��C in terms of decrease in leaf water content, increased electrolyte leakage and lipid peroxidation, decreased chlorophyll content, increased accumulation of proline and total sugars, and increased antioxidative activity of superoxide dismutase, catalase, guaiacol peroxidase, ascorbate peroxidase, and glutathione reductase. The consequence of combined stress was more distinct than the individual heat stress and cannot be extrapolated from the synergistic effect of the two stresses. In addition, priming induced cross-tolerance at 35��C by improving the membrane damage, photorespiration, photosynthetic pigment, osmolyte accumulation, and some antioxidative enzymes. On the basis of all the above parameters, PDG4 was identified as the best performing variety and tolerant to heat stress while GPF2 was the worst performing and sensitive to heat stress. It can be inferred that both severity and duration of stress are important and priming can be considered as an important tool to induce cross-tolerance in crop plants. � 2022, The Author(s), under exclusive licence to Korean Society of Crop Science (KSCS).
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    Drought priming induces chilling tolerance and improves reproductive functioning in chickpea (Cicer arietinum L.)
    (Springer Science and Business Media Deutschland GmbH, 2022-08-02T00:00:00) Saini, Rashmi; Das, Rangman; Adhikary, Arindam; Kumar, Rashpal; Singh, Inderjit; Nayyar, Harsh; Kumar, Sanjeev
    Key message: Priming alleviates membrane damage, chlorophyll degradation along with cryoprotectants accumulation during chilling stress that leads to improved reproductive functioning and increased seed yield. Abstract: Chilling temperatures below 15��C have severe implications on the reproductive growth and development of chickpea. The abnormal reproductive development and subsequent reproductive failure lead to substantial yield loss. We exposed five chickpea cultivars (PBG1, GPF2, PDG3, PDG4, and PBG5) to drought stress (Priming) during the vegetative stage and analyzed for chilling tolerance during the reproductive stage. These varieties were raised in the fields in two sets: one set of plants were subjected to drought stress at the vegetative stage for 30�days (priming) and the second set of plants were irrigated regularly (non-primed). The leaf samples were harvested at the flowering, podding, and seed filling stage and analyzed for membrane damage, water status, chlorophyll content, cellular respiration, and certain cryoprotective solutes. The reproductive development was analyzed by accessing pollen viability, in vivo and in vitro germination, pollen load, and in vivo pollen tube growth. Principal component analysis (PCA) revealed that priming improved membrane damage, chlorophyll b degradation, and accumulation of cryoprotectants in GPF2, PDG3, and PBG5 at the flowering stage (< 15��C). Pearson's correlation analysis showed a negative correlation with the accumulation of proline and carbohydrates with flower, pod, and seed abortion. Only, PBG5 responded best to priming while PBG1 was worst. In PBG5, priming resulted in reduced membrane damage and lipid peroxidation, improved water content, reduced chlorophyll degradation, and enhanced cryoprotective solutes accumulation, which led to increased reproductive functioning and finally improved seed yield and harvest index. Lastly, the priming response is variable and cultivar-specific but overall improve plant tolerance. � 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
  • No Thumbnail Available
    Item
    Understanding cross-tolerance mechanism and effect of drought priming on individual heat stress and combinatorial heat and drought stress in chickpea
    (Springer, 2022-03-15T00:00:00) Yadav, Renu; Juneja, Sumandeep; Kumar, Rashpal; Saini, Rashmi; Kumar, Sanjeev
    In northern India, chickpea experiences abrupt heat and drought stress during the late developmental stage and entails significant production loss. In the present study, the effect of heat stress and combined stress (heat and drought) was assessed in five varieties of chickpea along with the underlying mechanism of cross-tolerance by priming. Healthy seedlings (24�days old) were primed with mild drought stress and exposed to three different temperatures, i.e., 38��C, 35��C, and 32��C for 12, 24, and 36�h respectively, and also in combination with drought. The damage and tolerance were evaluated based on biochemical and physiological indicators. Results indicate that significant response was observed at 35��C as compared to 32 and 38��C in terms of decrease in leaf water content, increased electrolyte leakage and lipid peroxidation, decreased chlorophyll content, increased accumulation of proline and total sugars, and increased antioxidative activity of superoxide dismutase, catalase, guaiacol peroxidase, ascorbate peroxidase, and glutathione reductase. The consequence of combined stress was more distinct than the individual heat stress and cannot be extrapolated from the synergistic effect of the two stresses. In addition, priming induced cross-tolerance at 35��C by improving the membrane damage, photorespiration, photosynthetic pigment, osmolyte accumulation, and some antioxidative enzymes. On the basis of all the above parameters, PDG4 was identified as the best performing variety and tolerant to heat stress while GPF2 was the worst performing and sensitive to heat stress. It can be inferred that both severity and duration of stress are important and priming can be considered as an important tool to induce cross-tolerance in crop plants. � 2022, The Author(s), under exclusive licence to Korean Society of Crop Science (KSCS).