Department Of Botany

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Author: search.filters.author.Yadav, Renu

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    Drought priming evokes essential regulation of Hsp gene families, Hsfs and their related miRNAs and induces heat stress tolerance in chickpea
    (Elsevier B.V., 2023-07-26T00:00:00) Juneja, Sumandeep; Saini, Rashmi; Adhikary, Arindam; Yadav, Renu; Khan, Shahied Ahmed; Nayyar, Harsh; Kumar, Sanjeev
    Optimum temperature is crucial for plant's survival. During high temperature stress, heat shock proteins (Hsps) are expressed many folds essentially controlled by explicit heat shock factors (Hsfs).We have narrowed key HSPs, related HSFs and miRNAs regulated after priming with drought stress and consequent heat stress in chickpea. Firstly, we identified Hsf and Hsp gene families in desi and kabuli chickpea using Genome-wide analysis. Thereafter, selected Hsfs, Hsps and related miRNAs were analyzed using qRT-PCR in contrasting chickpea varieties (PBG1 and PBG5) after drought priming and exposing at 32 �C 24 hrs, 35 �C 12 hrs, and 38 �C 6 hrs. An interaction network between Hsfs and Hsps was generated. 18 & 17 Hsfs and 42 & 34 Hsps were identified in the desi and kabuli, respectively. The gene structure and motif composition of the genes were found to be conserved in all subfamilies. A total of 32 heat shock genes were found to have undergone duplication. Most of the CaHsf and CaHsp genes were differentially expressed on exposure to a combination of drought priming and heat stress in both in-silico and qPCR analysis. Targeted miRNAs expression was coordinated with the respective genes. miR156, miR166, miR319, miR171, and miR5213 were identified to be targets of sHsps, Hsfs, and Hsps. The protein-protein interaction revealed that CaHsp18.2 and CaHsp70 might be controlled by CaHsfsA1. Drought priming strongly correlated with less membrane damage and better leaf water content. Higher harvest index and root shoot ratio significantly indicated effectiveness of priming and essential role of Hsf and Hsp and related miRNAs in heat stress tolerance. � 2023
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    Understanding cross-tolerance mechanism and effect of drought priming on individual heat stress and combinatorial heat and drought stress in chickpea
    (Springer, 2022-03-15T00:00:00) Yadav, Renu; Juneja, Sumandeep; Kumar, Rashpal; Saini, Rashmi; Kumar, Sanjeev
    In northern India, chickpea experiences abrupt heat and drought stress during the late developmental stage and entails significant production loss. In the present study, the effect of heat stress and combined stress (heat and drought) was assessed in five varieties of chickpea along with the underlying mechanism of cross-tolerance by priming. Healthy seedlings (24�days old) were primed with mild drought stress and exposed to three different temperatures, i.e., 38��C, 35��C, and 32��C for 12, 24, and 36�h respectively, and also in combination with drought. The damage and tolerance were evaluated based on biochemical and physiological indicators. Results indicate that significant response was observed at 35��C as compared to 32 and 38��C in terms of decrease in leaf water content, increased electrolyte leakage and lipid peroxidation, decreased chlorophyll content, increased accumulation of proline and total sugars, and increased antioxidative activity of superoxide dismutase, catalase, guaiacol peroxidase, ascorbate peroxidase, and glutathione reductase. The consequence of combined stress was more distinct than the individual heat stress and cannot be extrapolated from the synergistic effect of the two stresses. In addition, priming induced cross-tolerance at 35��C by improving the membrane damage, photorespiration, photosynthetic pigment, osmolyte accumulation, and some antioxidative enzymes. On the basis of all the above parameters, PDG4 was identified as the best performing variety and tolerant to heat stress while GPF2 was the worst performing and sensitive to heat stress. It can be inferred that both severity and duration of stress are important and priming can be considered as an important tool to induce cross-tolerance in crop plants. � 2022, The Author(s), under exclusive licence to Korean Society of Crop Science (KSCS).
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    Unravelling cross priming induced heat stress, combinatorial heat and drought stress response in contrasting chickpea varieties
    (Elsevier Masson s.r.l., 2022-04-01T00:00:00) Yadav, Renu; Saini, Rashmi; Adhikary, Arindam; Kumar, Sanjeev
    Drought and high temperature stress affect chickpea growth and productivity. Often these stresses occur simultaneously in the field and lead to a wide range of molecular and metabolic adaptations. Two chickpea varieties; GPF2 (heat sensitive) and PDG4 variety (heat tolerant) were exposed to 35 �C for 24 h individually and along with drought stress. Five heat responsive signalling genes and 11 structural genes were analyzed using qPCR along with untargeted metabolites analysis using GC MS. Expression of antioxidant genes (CaSOD and CaGPX, CaAPX and CaCAT), transcription factors (CaHSFB2, CaHSFB2A, CaHSFB2B, CaHSP17.5 and CaHSP22.7) and signalling genes (CaCAM, CaGAD, and CaMAPK) were upregulated in GPF2 as compared to PDG4 variety. Principal component analysis (PCA), partial least-square discriminant analysis (PLS-DA), and heat map analysis were applied to the metabolomics data to identify the differential response of metabolites in two chickpea varieties. GC-MS analysis identified 107 and 83 metabolites in PDG4 and GPF2 varieties respectively. PDG4 variety accumulated more sugars, amino acids, sugar alcohols, TCA cycle intermediates which provided heat resistance. Additionally, the differential metabolic pathways involved in heat tolerance were alanine, aspartate, and glutamate metabolism, pantothenate CoA biosynthesis, fructose and mannose metabolism and pentose phosphate pathway in PDG4 variety. There was less accumulation of metabolites in the primed plants of both varieties as compared to the non-primed plants indicating less damage due to heat stress. The present study gives an overview of the molecular changes occurring in response to heat stress in sensitive and tolerant chickpea. � 2022 Elsevier Masson SAS
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    Drought priming evokes essential regulation of Hsp gene families, Hsfs and their related miRNAs and induces heat stress tolerance in chickpea
    (Elsevier B.V., 2023-07-26T00:00:00) Juneja, Sumandeep; Saini, Rashmi; Adhikary, Arindam; Yadav, Renu; Khan, Shahied Ahmed; Nayyar, Harsh; Kumar, Sanjeev
    Optimum temperature is crucial for plant's survival. During high temperature stress, heat shock proteins (Hsps) are expressed many folds essentially controlled by explicit heat shock factors (Hsfs).We have narrowed key HSPs, related HSFs and miRNAs regulated after priming with drought stress and consequent heat stress in chickpea. Firstly, we identified Hsf and Hsp gene families in desi and kabuli chickpea using Genome-wide analysis. Thereafter, selected Hsfs, Hsps and related miRNAs were analyzed using qRT-PCR in contrasting chickpea varieties (PBG1 and PBG5) after drought priming and exposing at 32 �C 24 hrs, 35 �C 12 hrs, and 38 �C 6 hrs. An interaction network between Hsfs and Hsps was generated. 18 & 17 Hsfs and 42 & 34 Hsps were identified in the desi and kabuli, respectively. The gene structure and motif composition of the genes were found to be conserved in all subfamilies. A total of 32 heat shock genes were found to have undergone duplication. Most of the CaHsf and CaHsp genes were differentially expressed on exposure to a combination of drought priming and heat stress in both in-silico and qPCR analysis. Targeted miRNAs expression was coordinated with the respective genes. miR156, miR166, miR319, miR171, and miR5213 were identified to be targets of sHsps, Hsfs, and Hsps. The protein-protein interaction revealed that CaHsp18.2 and CaHsp70 might be controlled by CaHsfsA1. Drought priming strongly correlated with less membrane damage and better leaf water content. Higher harvest index and root shoot ratio significantly indicated effectiveness of priming and essential role of Hsf and Hsp and related miRNAs in heat stress tolerance. � 2023
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    Understanding cross-tolerance mechanism and effect of drought priming on individual heat stress and combinatorial heat and drought stress in chickpea
    (Springer, 2022-03-15T00:00:00) Yadav, Renu; Juneja, Sumandeep; Kumar, Rashpal; Saini, Rashmi; Kumar, Sanjeev
    In northern India, chickpea experiences abrupt heat and drought stress during the late developmental stage and entails significant production loss. In the present study, the effect of heat stress and combined stress (heat and drought) was assessed in five varieties of chickpea along with the underlying mechanism of cross-tolerance by priming. Healthy seedlings (24�days old) were primed with mild drought stress and exposed to three different temperatures, i.e., 38��C, 35��C, and 32��C for 12, 24, and 36�h respectively, and also in combination with drought. The damage and tolerance were evaluated based on biochemical and physiological indicators. Results indicate that significant response was observed at 35��C as compared to 32 and 38��C in terms of decrease in leaf water content, increased electrolyte leakage and lipid peroxidation, decreased chlorophyll content, increased accumulation of proline and total sugars, and increased antioxidative activity of superoxide dismutase, catalase, guaiacol peroxidase, ascorbate peroxidase, and glutathione reductase. The consequence of combined stress was more distinct than the individual heat stress and cannot be extrapolated from the synergistic effect of the two stresses. In addition, priming induced cross-tolerance at 35��C by improving the membrane damage, photorespiration, photosynthetic pigment, osmolyte accumulation, and some antioxidative enzymes. On the basis of all the above parameters, PDG4 was identified as the best performing variety and tolerant to heat stress while GPF2 was the worst performing and sensitive to heat stress. It can be inferred that both severity and duration of stress are important and priming can be considered as an important tool to induce cross-tolerance in crop plants. � 2022, The Author(s), under exclusive licence to Korean Society of Crop Science (KSCS).
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    Unravelling cross priming induced heat stress, combinatorial heat and drought stress response in contrasting chickpea varieties
    (Elsevier Masson s.r.l., 2022-04-01T00:00:00) Yadav, Renu; Saini, Rashmi; Adhikary, Arindam; Kumar, Sanjeev
    Drought and high temperature stress affect chickpea growth and productivity. Often these stresses occur simultaneously in the field and lead to a wide range of molecular and metabolic adaptations. Two chickpea varieties; GPF2 (heat sensitive) and PDG4 variety (heat tolerant) were exposed to 35 �C for 24 h individually and along with drought stress. Five heat responsive signalling genes and 11 structural genes were analyzed using qPCR along with untargeted metabolites analysis using GC MS. Expression of antioxidant genes (CaSOD and CaGPX, CaAPX and CaCAT), transcription factors (CaHSFB2, CaHSFB2A, CaHSFB2B, CaHSP17.5 and CaHSP22.7) and signalling genes (CaCAM, CaGAD, and CaMAPK) were upregulated in GPF2 as compared to PDG4 variety. Principal component analysis (PCA), partial least-square discriminant analysis (PLS-DA), and heat map analysis were applied to the metabolomics data to identify the differential response of metabolites in two chickpea varieties. GC-MS analysis identified 107 and 83 metabolites in PDG4 and GPF2 varieties respectively. PDG4 variety accumulated more sugars, amino acids, sugar alcohols, TCA cycle intermediates which provided heat resistance. Additionally, the differential metabolic pathways involved in heat tolerance were alanine, aspartate, and glutamate metabolism, pantothenate CoA biosynthesis, fructose and mannose metabolism and pentose phosphate pathway in PDG4 variety. There was less accumulation of metabolites in the primed plants of both varieties as compared to the non-primed plants indicating less damage due to heat stress. The present study gives an overview of the molecular changes occurring in response to heat stress in sensitive and tolerant chickpea. � 2022 Elsevier Masson SAS
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    Preconditioning of chickpea seedlings for terminal heat stress: understanding associated mechanism and HSP's expression
    (Central University of Punjab, 2014) Yadav, Renu; Kumar, Sanjeev
    Chickpea (Cicer arietinium L.) is the second most important pulse crop grown worldwide. Changes in the cropping system, competition from other cash crops (wheat) and global warming are pushing chickpea to relatively warmer growing environment. In northern part of country chickpea come across with terminal high temperature stress during reproductive stage which lead to reduced grain yield. Therefore to prevent the plant from incoming heat stress, 11 day chickpea seedling were preconditioned with mild drought stress, then put on recovery for six days and then recovered seedlings were exposed to lethal stress (where temperature was increased step wise from 30?C to 36?C). This study revealed that % EL, Lipid peroxidation increased with the increase in temperature while percent TTC reduction and total protein content decreased with the increase in temperature. Antioxidative enzymes provide the major defence against the ROS generated during the abiotic stress, and it was found that activity of SOD, CAT and APX enzyme increased proportionately with the rising temperature. HSP's act as molecular chaperons and are over expressed at both mRNA and protein level in preconditioned seedlings exposed to high temperature stress as compared to non- preconditioned ones. To conclude the whole study, results obtained clearly reveal that preconditioning with drought stress has the ability to improve tolerance above ambient temperature (27?C 7?C), thereafter preconditioning did not have any influence in terms of the improvement in membrane damage and level of antioxidants. Higher expression of sHSP's is corroborated with the low expression of antioxidants.
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    Drought and heat tolerance in chickpea: Transcriptome and morphophysiological changes under individual and combined stress
    (Springer India, 2017) Yadav, Renu; Juneja, Sumandeep; Singh, Priyanka; Kumar, Sanjeev
    Increase in global temperature due to climate change is the major concern and known to have detrimental effect on many agricultural crops. Chickpea (Cicer arietinum L.) is an important legume grown in the arid and semiarid region of the world. Chickpea being a heat sensitive crop is greatly affected by heat stress during both vegetative and reproductive stages. Stress resistance mechanism of chickpea involves signal perception, transduction, and subsequent activation of stress-responsive genes encoding reactive oxygen species (ROS) scavenging and osmolyte, chaperones, and aquaporins. There are different stress perception and signaling pathways, both in drought and high-temperature stress, but some common pathways also exist between the two mechanisms. The present chapter summarizes the cross talk between the drought and heat stress and the molecular mechanism underlying individual stress. Field plants are exposed to multiple stresses, and the combined effect might be antagonistic or synergistic. Hence, improving stress tolerance of plants requires a reevaluation, taking into account the effect of multiple stresses on plant metabolism and stress resistance. ? Springer (India) Pvt. Ltd. 2017. All rights reserved.