Department Of Botany

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    First report of Planomicrobium okeanokoites associated with Himantothallus grandifolius (Desmarestiales, Phaeophyta) from Southern Hemisphere
    (Public Library of Science, 2023-04-14T00:00:00) Chand Saini, Khem; Gupta, Kriti; Sharma, Sheetal; Gautam, Ajay K.; Shamim, Samrin; Mittal, Divya; Kundu, Pushpendu; Bast, Felix
    Gram-positive, aerobic, motile, rod-shaped, mesophilic epiphytic bacterium Planomicrobium okeanokoites was isolated from the surface of endemic species Himantothallus grandifolius in Larsemann Hills, Eastern Antarctica. The diversity of epiphytic bacterial communities living on marine algae remains primarily unexplored; virtually no reports from Antarctic seaweeds. The present study used morpho-molecular approaches for the macroalgae and epiphytic bacterium characterization. Phylogenetic analysis was performed using mitochondrial genome encoded COX1 gene; chloroplast genome encodes rbcL; nuclear genome encoded large subunit ribosomal RNA gene (LSU rRNA) for Himantothallus grandifolius and ribosomal encoded 16S rRNA for Planomicrobium okeanokoites. Morphological and molecular data revealed that the isolate is identified as Himantothallus grandifolius, which belongs to Family Desmarestiaceae of Order Desmarestiales in Class Phaeophyceae showing 99.8% similarity to the sequences of Himantothallus grandifolius, from King George Island, Antarctica (HE866853). The isolated bacterial strain was identified on the basis of chemotaxonomic, morpho-phylogenetic, and biochemical assays. A phylogenetic study based on 16S rRNA gene sequences revealed that the epiphytic bacterial strain SLA-357 was closest related to the Planomicrobium okeanokoites showing 98.7% sequence similarity. The study revealed the first report of this species from the Southern Hemisphere to date. Also, there has been no report regarding the association between the Planomicrobium okeanokoites and Himantothallus grandifolius; however, there are some reports on this bacterium isolated from sediments, soils, and lakes from Northern Hemisphere. This study may open a gateway for further research to know about the mode of interactions and how they affect the physiology and metabolism of each other. � 2023 Saini et al.
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    First report of Planomicrobium okeanokoites associated with Himantothallus grandifolius (Desmarestiales, Phaeophyta) from Southern Hemisphere
    (Public Library of Science, 2023-04-14T00:00:00) Chand Saini, Khem; Gupta, Kriti; Sharma, Sheetal; Gautam, Ajay K.; Shamim, Samrin; Mittal, Divya; Kundu, Pushpendu; Bast, Felix
    Gram-positive, aerobic, motile, rod-shaped, mesophilic epiphytic bacterium Planomicrobium okeanokoites was isolated from the surface of endemic species Himantothallus grandifolius in Larsemann Hills, Eastern Antarctica. The diversity of epiphytic bacterial communities living on marine algae remains primarily unexplored; virtually no reports from Antarctic seaweeds. The present study used morpho-molecular approaches for the macroalgae and epiphytic bacterium characterization. Phylogenetic analysis was performed using mitochondrial genome encoded COX1 gene; chloroplast genome encodes rbcL; nuclear genome encoded large subunit ribosomal RNA gene (LSU rRNA) for Himantothallus grandifolius and ribosomal encoded 16S rRNA for Planomicrobium okeanokoites. Morphological and molecular data revealed that the isolate is identified as Himantothallus grandifolius, which belongs to Family Desmarestiaceae of Order Desmarestiales in Class Phaeophyceae showing 99.8% similarity to the sequences of Himantothallus grandifolius, from King George Island, Antarctica (HE866853). The isolated bacterial strain was identified on the basis of chemotaxonomic, morpho-phylogenetic, and biochemical assays. A phylogenetic study based on 16S rRNA gene sequences revealed that the epiphytic bacterial strain SLA-357 was closest related to the Planomicrobium okeanokoites showing 98.7% sequence similarity. The study revealed the first report of this species from the Southern Hemisphere to date. Also, there has been no report regarding the association between the Planomicrobium okeanokoites and Himantothallus grandifolius; however, there are some reports on this bacterium isolated from sediments, soils, and lakes from Northern Hemisphere. This study may open a gateway for further research to know about the mode of interactions and how they affect the physiology and metabolism of each other. � 2023 Saini et al.
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    Isolation And Culturing Of Photobionts From Antarctic Lichen
    (Central University of Punjab, 2018) Acharya, Sayanya; Bast, Felix
    Lichen is the symbiotic association in between algal and fungal partner. Lichens having various economic importance. The main objective is to isolate and culture of the photobionts from the lichen samples collected from Antarctica. Trebouxia sp. is extremely psychrophilic and fastidious in growth, found in the lichen samples. Both samples belong to genus Umbilicaria, Umbilicaria antarctica and Umbilicaria aprina. In the lichen samples Gyrophoric acid is found as secondary metabolites. First to isolate the photobionts, we use micromethod and for the culturing use 3M BBM media for 45-60 days at 22-25o C temperature. Then subculturing process is done and followed by DNA extraction. The extracted DNA was kept for further use in sequencing purpose. The isolated and cultured photobionts can be further studied using DNA sequencing, SEM, and confocal microscopy to get a clear idea about the photobionts and its internal organelles; these will help in proper identification of the photobionts as well as the lichen. Further phylogenetic studies of photobionts and coevolutionary studies between photobionts and mycobionts which may be done by extensive taxa sampling and through use of molecular systematics techniques.