Theses And Dissertation
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Item Synthesis And Antiproliferative Activity Of Pyrazole-Based Heterocycles(Central University of Punjab, 2018) Pandey, Vishakha; Kumar, RajAmong the various heterocyclic compounds pyrazole and its derivatives have occupied wide range of biological and pharmacological activities. These were observed for their modes of function in the inhibition of topoisomerase and DNA repair. DNA topoisomerases usually modify DNA topology by their ability to break and reseals both its strands. Which were leads to DNA replication, transcription processes. It helps as a vital targets for numerous chemotherapeutic agents. The potency of topoisomerase inhibitors looks to be diminishing due to drug resistance and lack of efficacy. Thus, after long glimpsing the current scenario was made in order to develop topoisomerase inhibitors with completely new scaffold or alteration or modification in the existing scaffold. We herein report design and synthesis of pyrazole based compounds as topoisomerase inhibitors. The synthetics were evaluated for their in vitro anticancer activity against MDAMB 231 breast cancer cell line.Item Oxidative stress responses to sub-lethal dose of Cry toxin in the larvae of castor semilooper, Achaea janata(Central University of Punjab, 2018) Singh, Kanika; Chaitanya,R.K.Development of synthetic insecticides to reduce the level of infestation led to deleterious effects on environment and human health. This lead to the development of ecofriendly pest management alternatives including Bacillus thuringensis (Bt). Bt produce Crystal (Cry), Cytotoxic (Cyt) and Vegetative (Vip) proteins with insecticidal activity against different orders of lepidoptera. Of late, pest resistance against Bt is reported in countries.The reduced toxicity of Bt formulation from degradation by UV light, wash-off by rain, drying, temperature, and soil acidity as well as its chemistry. Further, insects sense pesticides through odorant receptors and move away quickly, there is always a possibility of a population of larvae to get exposed to sub-lethal doses of toxin which might exhibit variable effects and escape mortality and eventually generate resistance. Sub-lethal dose lead to the generation of oxidative stress in the insect and eventually scavenged by anti-oxidant enzymes. These stress responses would enhance our understanding of adaptations for survival and resistance development. The current study is an attempt to monitor the antioxidative responses at the transcriptional level upon sub-lethal exposure of Cry toxin in the larvae of an polyphagous pest castor semilooper, Achaea janata. prevalent in the Indian subcontinent.Item Role of midgut digestive proteases upon sub-lethal exposure of Cry toxin in the larvae of castor semilooper, Achaea janata(Central University of Punjab, 2018) Sureshgopi, D; Chaitanya, R.K.Development of synthetic insecticides to reduce the level of infestation led to deleterious effects on the environment and human health. This lead to the development of eco-friendly pest management alternatives including Bacillus thuringensis (Bt). Bt produces Crystal (Cry), Cytotoxic (Cyt) and Vegetative (Vip) proteins with insecticidal activity against different orders of Lepidoptera. Of late, pest resistance against Bt is being reported in many parts of the country. The foliar spray may lead to the reduced toxicity of Bt formulation from degradation by UV light, wash-off by rain, drying, temperature, and soil acidity as well as its chemistry. Further, insects sense pesticides through odorant receptors and move away quickly. Under these conditions, there is always a possibility of a population of larvae to get exposed to sub-lethal doses of toxin which might exhibit variable effects and escape mortality and eventually generate resistance. The role played by digestive proteases in the activation of the Cry toxin and digestion when administered in sub-lethal concentration could help in the elucidation of key phenomena like adaptations for survival and resistance development. Elucidation of these digestive proteases would enhance our understanding of resistance development. The current study is an attempt to monitor the transcript levels of digestive proteases upon sub-lethal exposure of Cry toxin in the larvae of an economically important polyphagous pest castor semilooper, Achaea janata prevalent in the Indian subcontinent.