Self assembled monolayer based liquid crystal biosensor for free cholesterol detection
Files
Date
2014, 2014
Journal Title
Journal ISSN
Volume Title
Publisher
American Institute of Physics Inc.
Abstract
A unique cholesterol oxidase (ChOx) liquid crystal (LC) biosensor, based on the disruption of orientation in LCs, is developed for cholesterol detection. A self-assembled monolayer (SAM) of Dimethyloctadecyl[3-(trimethoxysilyl)propyl] ammonium chloride (DMOAP) and (3-Aminopropyl)trimethoxy-silane (APTMS) is prepared on a glass plate by adsorption. The enzyme (ChOx) is immobilized on SAM surface for 12 h before utilizing the film for biosensing purpose. LC based biosensing study is conducted on SAM/ChOx/LC (5CB) cells for cholesterol concentrations ranging from 10mg/dl to 250 mg/dl. The sensing mechanism has been verified through polarizing optical microscopy, scanning electron microscopy, and spectrometric techniques. ? 2014 AIP Publishing LLC.
Description
Keywords
Chlorine compounds, Cholesterol, Liquid crystals, Scanning electron microscopy, Self assembled monolayers, Ammonium chloride, Cholesterol detection, Cholesterol oxidase, Free cholesterols, Liquid crystal biosensors, Polarizing optical microscopy, Sensing mechanism, Spectrometric techniques, Biosensors
Citation
Tyagi, M., Chandran, A., Joshi, T., Prakash, J., Agrawal, V. V., & Biradar, A. M. (2014). Self assembled monolayer based liquid crystal biosensor for free cholesterol detection. Applied Physics Letters, 104(15). doi: 10.1063/1.4871704