Department Of Botany

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Now showing 1 - 6 of 6
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    Genome-wide identification and gene expression analysis of GHMP kinase gene family in banana cv. Rasthali
    (Springer Science and Business Media B.V., 2023-09-20T00:00:00) Chaturvedi, Siddhant; Khan, Shahirina; Thakur, Neha; Jangra, Alka; Tiwari, Siddharth
    Background: The GHMP kinase gene family encompasses ATP-dependent kinases, significantly involved in the biosynthesis of isoprenes, amino acids, and metabolism of carbohydrates. Banana is a staple tropical crop that is globally consumed but known for high sensitivity to salt, cold, and drought stresses. The GHMP kinases are known to play a significant role during abiotic stresses in plants. The present study emphasizes the role of GHMP kinases in various abiotic stress conditions in banana. Methods and results: We identified 12 GHMP kinase (MaGHMP kinase) genes in the banana genome database and witnessed the presence of the conserved Pro-X-X-X-Gly-Leu-X-Ser-Ser-Ala domain in their protein sequences. All genes were found to be involved in ATP-binding and carried kinase activity confronting their biological roles in the isoprene (27%) and amino acid (20%) biosyntheses. The expression analysis of genes during cold, drought, and salt stress conditions in tissue culture grown banana cultivar Rasthali plants showed a significant involvement of MaGHMP kinase genes in these stress conditions. The highest expression of MaGHMP kinase3 (8.5 fold) was noted during cold stress, while MaGHMP kinase1 (25 fold and 40.01 fold) showed maximum expression during drought and salt stress conditions in leaf tissue of Rasthali. Conclusion: Our findings suggested that MaGHMP kinase1 (MaHSK) and MaGHMP kinase3 (MaGlcAK) could be considered promising candidates for thwarting the abiotic stresses in banana. � 2023, The Author(s), under exclusive licence to Springer Nature B.V.
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    Analysis of TCP Transcription Factors Revealed Potential Roles in Plant Growth and Fusarium oxysporum f.sp. cubense Resistance in Banana (cv. Rasthali)
    (Springer, 2022-07-05T00:00:00) Chaturvedi, Siddhant; Khan, Shahirina; Usharani, T.R.; Tiwari, Siddharth
    The TCP transcription factor gene family is highly conserved among the plant species. It plays a major role in the regulation of flower symmetry, cell division, and development of leaf, fibre, and nodule in the plants by controlling the synthesis of various plant hormones. Banana is a major staple crop in the world. However, Fusarium oxysporum f. sp. cubense (Foc) infection is a major threat to banana production. The role of TCP gene family during the Foc infection is not explored till now. Herein, a total of 27 non-redundant TCP (MaTCP) gene sequences were retrieved from the banana genome and analysed for structural characteristics, phylogenetic correlation, subcellular, and chromosomal localizations. Phylogenetic analysis showed that the MaTCP proteins were highly conserved among different species and found to be the closest relative of the Oryza sativa and Zea mays. Promoter analysis of the TCP sequences showed that the cis-acting regulatory elements are associated with various stresses and environmental and hormonal signals. The higher transcript accumulation in developing tissues (fruit finger, leaves, and stem) than of mature tissues (peel and pulp) showed a significant role of MaTCP in banana (cv. Rasthali) growth and development. Further, higher expression of the certain MaTCPs in Foc race 1-infected root (MaTCP2, MaTCP4, MaTCP6) and leaf (MaTCP9 and MaTCP11) tissues of Rasthali indicated their promising role during Fusarium infection. This study will underpin the facet of TCP transcription factors on the development of biotic (Foc) stress resistance in banana. � 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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    Transgene-free genome editing supports the role of carotenoid cleavage dioxygenase 4 as a negative regulator of ?-carotene in banana
    (Oxford University Press, 2022-02-01T00:00:00) Awasthi, Praveen; Khan, Shahirina; Lakhani, Hiralben; Chaturvedi, Siddhant; Shivani, S.; Kaur, Navneet; Singh, Jagdeep; Kesarwani, Atul Kumar; Tiwari, Siddharth
    Carotenoid cleavage dioxygenases (CCDs) belong to a small gene family and have an important role in the intricate metabolism of carotenoids in plants. In this study we aimed to understand the regulatory mechanism of ?-carotene homeostasis by establishing transgene-free genome editing in banana. We found that the expression patterns of multiple CCDs were correlated with the concentrations of carotenoids in two cultivars with contrasting contents of ?-carotene, Nendran (high) and Rasthali (low). Higher expression of CCD4 in Rasthali (RAS-CCD4) was negatively correlated with ?-carotene accumulation in the fruit-pulp. Docking analysis and enzyme assays of purified RAS-CCD4 suggested that ?-carotene and 10-Apo-?-carotenal were the preferred substrates of RAS-CCD4. Bacterial complementation assays demonstrated the role of RAS-CCD4 in ?-carotene degradation, and this was further confirmed by in vivo overexpression of RAS-CCD4 in Arabidopsis, which resulted in significant reduction in ?-carotene concentration. CRISPR/Cas9-mediated editing of CCD4 was conducted in protoplasts and embryogenic cell lines of Rasthali, and carotenoid profiling in the resulting stable mutant lines showed greater increases in ?-carotene accumulation in the roots than in the leaves compared with unedited controls. The differences in expression of carotenoid pathway genes were correlated with differences in metabolites in the edited lines. Overall, our study suggests that carotenoid catabolism is regulated by CCD4 in ways that are tissue-and cultivar-specific, and it also demonstrates the successful use of the genome-editing tool in developing transgene-free biofortified lines of banana. � 2022 The Author(s) 2022.
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    Genome-wide identification and gene expression analysis of GHMP kinase gene family in banana cv. Rasthali
    (Springer Science and Business Media B.V., 2023-09-20T00:00:00) Chaturvedi, Siddhant; Khan, Shahirina; Thakur, Neha; Jangra, Alka; Tiwari, Siddharth
    Background: The GHMP kinase gene family encompasses ATP-dependent kinases, significantly involved in the biosynthesis of isoprenes, amino acids, and metabolism of carbohydrates. Banana is a staple tropical crop that is globally consumed but known for high sensitivity to salt, cold, and drought stresses. The GHMP kinases are known to play a significant role during abiotic stresses in plants. The present study emphasizes the role of GHMP kinases in various abiotic stress conditions in banana. Methods and results: We identified 12 GHMP kinase (MaGHMP kinase) genes in the banana genome database and witnessed the presence of the conserved Pro-X-X-X-Gly-Leu-X-Ser-Ser-Ala domain in their protein sequences. All genes were found to be involved in ATP-binding and carried kinase activity confronting their biological roles in the isoprene (27%) and amino acid (20%) biosyntheses. The expression analysis of genes during cold, drought, and salt stress conditions in tissue culture grown banana cultivar Rasthali plants showed a significant involvement of MaGHMP kinase genes in these stress conditions. The highest expression of MaGHMP kinase3 (8.5 fold) was noted during cold stress, while MaGHMP kinase1 (25 fold and 40.01 fold) showed maximum expression during drought and salt stress conditions in leaf tissue of Rasthali. Conclusion: Our findings suggested that MaGHMP kinase1 (MaHSK) and MaGHMP kinase3 (MaGlcAK) could be considered promising candidates for thwarting the abiotic stresses in banana. � 2023, The Author(s), under exclusive licence to Springer Nature B.V.
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    Analysis of TCP Transcription Factors Revealed Potential Roles in Plant Growth and Fusarium oxysporum f.sp. cubense Resistance in Banana (cv. Rasthali)
    (Springer, 2022-07-05T00:00:00) Chaturvedi, Siddhant; Khan, Shahirina; Usharani, T.R.; Tiwari, Siddharth
    The TCP transcription factor gene family is highly conserved among the plant species. It plays a major role in the regulation of flower symmetry, cell division, and development of leaf, fibre, and nodule in the plants by controlling the synthesis of various plant hormones. Banana is a major staple crop in the world. However, Fusarium oxysporum f. sp. cubense (Foc) infection is a major threat to banana production. The role of TCP gene family during the Foc infection is not explored till now. Herein, a total of 27 non-redundant TCP (MaTCP) gene sequences were retrieved from the banana genome and analysed for structural characteristics, phylogenetic correlation, subcellular, and chromosomal localizations. Phylogenetic analysis showed that the MaTCP proteins were highly conserved among different species and found to be the closest relative of the Oryza sativa and Zea mays. Promoter analysis of the TCP sequences showed that the cis-acting regulatory elements are associated with various stresses and environmental and hormonal signals. The higher transcript accumulation in developing tissues (fruit finger, leaves, and stem) than of mature tissues (peel and pulp) showed a significant role of MaTCP in banana (cv. Rasthali) growth and development. Further, higher expression of the certain MaTCPs in Foc race 1-infected root (MaTCP2, MaTCP4, MaTCP6) and leaf (MaTCP9 and MaTCP11) tissues of Rasthali indicated their promising role during Fusarium infection. This study will underpin the facet of TCP transcription factors on the development of biotic (Foc) stress resistance in banana. � 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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    Transgene-free genome editing supports the role of carotenoid cleavage dioxygenase 4 as a negative regulator of ?-carotene in banana
    (Oxford University Press, 2022-02-01T00:00:00) Awasthi, Praveen; Khan, Shahirina; Lakhani, Hiralben; Chaturvedi, Siddhant; Shivani, S.; Kaur, Navneet; Singh, Jagdeep; Kesarwani, Atul Kumar; Tiwari, Siddharth
    Carotenoid cleavage dioxygenases (CCDs) belong to a small gene family and have an important role in the intricate metabolism of carotenoids in plants. In this study we aimed to understand the regulatory mechanism of ?-carotene homeostasis by establishing transgene-free genome editing in banana. We found that the expression patterns of multiple CCDs were correlated with the concentrations of carotenoids in two cultivars with contrasting contents of ?-carotene, Nendran (high) and Rasthali (low). Higher expression of CCD4 in Rasthali (RAS-CCD4) was negatively correlated with ?-carotene accumulation in the fruit-pulp. Docking analysis and enzyme assays of purified RAS-CCD4 suggested that ?-carotene and 10-Apo-?-carotenal were the preferred substrates of RAS-CCD4. Bacterial complementation assays demonstrated the role of RAS-CCD4 in ?-carotene degradation, and this was further confirmed by in vivo overexpression of RAS-CCD4 in Arabidopsis, which resulted in significant reduction in ?-carotene concentration. CRISPR/Cas9-mediated editing of CCD4 was conducted in protoplasts and embryogenic cell lines of Rasthali, and carotenoid profiling in the resulting stable mutant lines showed greater increases in ?-carotene accumulation in the roots than in the leaves compared with unedited controls. The differences in expression of carotenoid pathway genes were correlated with differences in metabolites in the edited lines. Overall, our study suggests that carotenoid catabolism is regulated by CCD4 in ways that are tissue-and cultivar-specific, and it also demonstrates the successful use of the genome-editing tool in developing transgene-free biofortified lines of banana. � 2022 The Author(s) 2022.