School Of Basic And Applied Sciences
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Item Synergistic effect of eco-friendly pistachio shell biomass on nano-MnO2 for crystal violet removal: kinetic and equilibrium studies(Institute for Ionics, 2022-05-07T00:00:00) Kumar, S.; Brar, R. Singh; Saha, S.; Dahiya, A.; Kalpana; Babu, J. NagendraPistachio shell powder-supported MnO2 nanostructure-based eco-friendly nanocomposite (nMPP) was prepared via one-pot redox precipitation method and was characterized by FTIR, XRD, SEM, TEM, BET, TGA/DTA, and XPS techniques. SEM and TEM analysis revealed the pseudo-spherical and nanorod morphologies of the synthesized nano-MnO2 and found agglomerated on the pistachio biomass. The nMPP contains nearly 41% Mn as MnO2 (w/w %) dispersed onto the pistachio shell biomass as confirmed from EDX, TGA, and AAS analysis. The nMPP exhibits multi-process crystal violet (CV) removal phenomenon under different pH of aqueous dye solution. Under acidic pH, nMPP caused oxidative degradation of CV by in situ formed.OH radicals; while under the neutral pH, CV undergoes monolayer adsorption onto the surface of nMPP as confirmed from Langmuir adsorption isotherm fit with maximum equilibrium adsorption value of 148.7�mg.g?1. The nMPP nanomaterial exhibits a synergistic effect between adsorption efficiencies of pistachio shell biomass and nano-MnO2 for the effective removal of toxic CV dye. The maximum saturation adsorption and rate constant (k 2) value obtained from the pseudo-second-order kinetic fit model were 119.13�mg.g?1 and 5.0 � 10�4�g.mg?1�min?1, respectively. Graphical abstract: [Figure not available: see fulltext.] � 2022, The Author(s) under exclusive licence to Iranian Society of Environmentalists (IRSEN) and Science and Research Branch, Islamic Azad University.Item Bacillus subtilis CP4, isolated from native soil in combination with arbuscular mycorrhizal fungi promotes biofortification, yield and metabolite production in wheat under field conditions(John Wiley and Sons Inc, 2020-12-03T00:00:00) Yadav, R.; Ror, P.; Rathore, P.; Kumar, S.; Ramakrishna, W.Aims: The aim of this study was to identify the best combination of plant growth promoting bacteria (PGPB) and arbuscular mycorrhizal fungi (AMF) for biofortification and enhancing yield in wheat as well as improve soil health under field conditions. Another aim was to get insights into metabolite dynamics in plants treated with PGPB and AMF. Methods and Results: Different combinations of PGPB and AMF that gave good results in greenhouse study were used in a field study. The combined application of Bacillus subtilis CP4 (native PGPB) and AMF gave the best results with a significant increase in biomass, macronutrient and micronutrient content in wheat grains and improvement in yield-related parameters relative to the untreated control. PGPB and AMF treatment increased antioxidant enzymes and compounds and decreased the level of an oxidation marker. Metabolite profiling performed using Gas Chromatography�Mass Spectrometry (GC-MS) showed significant upregulation of specific organic acids, amino acids, sugars and sugar alcohols in plants treated with CP4 and AMF. The altered pathways due to CP4 and AMF inoculation mainly belong to carbohydrate and amino acid metabolism. A positive correlation was observed between some organic acids, sugars and amino acids with wheat growth and yield parameters. The activities of soil enzymes increased significantly with the best results shown by native PGPB and AMF combination. Conclusions: A native bacterial isolate Bacillus subtilis CP4 in combination with AMF showed exceptional ability for biofortification and yield enhancement under field conditions. The upregulation of a number of metabolites showed correlation plant growth promotion and nutrients. Significance and Impact of the Study: The combined application of native B. subtilis CP4 and AMF could offer a more sustainable approach for the development of a biofertilizer to enhance wheat nutrient content and production and soil health thereby advancing agriculture. � 2020 The Society for Applied MicrobiologyItem Bacillus subtilis CP4, isolated from native soil in combination with arbuscular mycorrhizal fungi promotes biofortification, yield and metabolite production in wheat under field conditions(John Wiley and Sons Inc, 2020-12-03T00:00:00) Yadav, R.; Ror, P.; Rathore, P.; Kumar, S.; Ramakrishna, W.Aims: The aim of this study was to identify the best combination of plant growth promoting bacteria (PGPB) and arbuscular mycorrhizal fungi (AMF) for biofortification and enhancing yield in wheat as well as improve soil health under field conditions. Another aim was to get insights into metabolite dynamics in plants treated with PGPB and AMF. Methods and Results: Different combinations of PGPB and AMF that gave good results in greenhouse study were used in a field study. The combined application of Bacillus subtilis CP4 (native PGPB) and AMF gave the best results with a significant increase in biomass, macronutrient and micronutrient content in wheat grains and improvement in yield-related parameters relative to the untreated control. PGPB and AMF treatment increased antioxidant enzymes and compounds and decreased the level of an oxidation marker. Metabolite profiling performed using Gas Chromatography�Mass Spectrometry (GC-MS) showed significant upregulation of specific organic acids, amino acids, sugars and sugar alcohols in plants treated with CP4 and AMF. The altered pathways due to CP4 and AMF inoculation mainly belong to carbohydrate and amino acid metabolism. A positive correlation was observed between some organic acids, sugars and amino acids with wheat growth and yield parameters. The activities of soil enzymes increased significantly with the best results shown by native PGPB and AMF combination. Conclusions: A native bacterial isolate Bacillus subtilis CP4 in combination with AMF showed exceptional ability for biofortification and yield enhancement under field conditions. The upregulation of a number of metabolites showed correlation plant growth promotion and nutrients. Significance and Impact of the Study: The combined application of native B. subtilis CP4 and AMF could offer a more sustainable approach for the development of a biofertilizer to enhance wheat nutrient content and production and soil health thereby advancing agriculture. � 2020 The Society for Applied MicrobiologyItem Identification of potential natural inhibitors of SARS-CoV2 main protease by molecular docking and simulation studies(Taylor and Francis Ltd., 2020) Gupta, S; Singh, A.K; Kushwaha, P.P; Prajapati, K.S; Shuaib, M; Senapati, S; Kumar, S.Coronaviruses are contagious pathogens primarily responsible for respiratory and intestinal infections. Research efforts to develop antiviral agents against coronavirus demonstrated the main protease (Mpro) protein may represent effective drug target. X-ray crystallographic structure of the SARS-CoV2 Mpro protein demonstrated the significance of Glu166, Cys141, and His41 residues involved in protein dimerization and its catalytic function. We performed in silico screening of compounds from Curcuma longa L. (Zingiberaceae family) against Mpro protein inhibition. Employing a combination of molecular docking, scoring functions, and molecular dynamics simulations, 267 compounds were screened by docking on Mpro crystallographic structure. Docking score and interaction profile analysis exhibited strong binding on the Mpro catalytic domain with compounds C1 (1E,6E)-1,2,6,7-tetrahydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-1,6-diene-3,5-dione) and C2 (4Z,6E)?1,5?dihydroxy?1,7?bis(4?hydroxyphenyl)hepta?4,6?dien?3?one as lead agents. Compound C1 and C2 showed minimum binding score (�9.08 and �8.07 kcal/mole) against Mpro protein in comparison to shikonin and lopinavir (? ?5.4 kcal/mole) a standard Mpro inhibitor. Furthermore, principal component analysis, free energy landscape and protein-ligand energy calculation studies revealed that these two compounds strongly bind to the catalytic core of the Mpro protein with higher efficacy than lopinavir, a standard antiretroviral of the protease inhibitor class. Taken together, this structure based optimization has provided lead on two natural Mpro inhibitors for further testing and development as therapeutics against human coronavirus. Communicated by Ramaswamy H. Sarma. � 2020, � 2020 Informa UK Limited, trading as Taylor & Francis Group.Item Terpenoids: Synthesis and biological activities(Nova Science Publisher Inc., 2018) Kushwaha, P.P.; Kumar, S.Terpenoid is the naturally occurring compound synthesized from isoprene units. Terpenoid plays a variety of functions in the growth and development of plants. Most of the terpenoid perform chemical communications and defense function in both biotic and abiotic environment. Terpenoid is classified into different groups such as monoterpene, diterpene, a sesquiterpene, triterpene, tetraterpene, and polyterpene. Terpenoids are reported for their excellent anti-platelet aggregation and anticancer activity. They regulate cellular signaling pathway, cell cycle phases and induce apoptosis. In this chapter, we mainly focused on the different class of the terpenoid, function and their biosynthesis in the plant system. ? 2018 Nova Science Publishers, Inc.Item Secondary metabolite and functional food components: Role in health and disease(Nova Science Publisher Inc., 2018) Kumar, S.Secondary Metabolites and Functional Food Components: Their Roles in Health and Disease consists of original chapters, provides updates to previous source material and acts as a unique source of information for all those interested in secondary metabolites and functional food components. The proposed publication focuses on the pharmaceutical and food industries, with an emphasis on their aspects pertaining to chemistry, nutrition sciences, biochemistry and biology. In the present book, the major secondary metabolites and the chemistry in conjunction with functional food components have been discussed. The book also deals with the role of secondary metabolites in human health and diseases. This book is specifically marketed towards graduate and master?s students. It deals with the knowledge and recent advancements in the field of secondary metabolites and functional food components as well as their importance in health and disease. Moreover, the book will also be very useful for the students preparing for various competitive examinations such as the CSIR, ICMR, DBT, and ICAR JRF/NET exams. ? 2018 by Nova Science Publishers, Inc. All rights reserved.Item Objective and subjective exercise(Nova Science Publishers, Inc., 2017) Sharma, A.K.; Kumar, S.[No abstract available]Item Introduction to secondary metabolites(Nova Science Publisher Inc., 2018) Kumar, S.Metabolic pathways in a microorganism, used for the production of energy, are known as primary metabolic pathway. The metabolic product or intermediary metabolites of primary metabolic pathway are used for the production of secondary metabolites. The pathway used for the production of secondary metabolites is known as secondary metabolism pathway. Secondary metabolites are defined as the chemical compounds that are not directly involved in the growth and development of an organism. Plants, animals, and microbes are known to produce secondary metabolites for various purposes such as defense from herbivores and predators, for pollination, to attract opposite sex individual of the same species, to invade host pathophysiological barriers, and protection from ultraviolet radiations, etc. Secondary metabolites are the basis of various medicinal system worlds around. Beside pharmaceutical industry secondary metabolites have an important place in textile, paper, cosmetics and other industries. Different techniques have been evolved from time to time with the advancement of science to increase the production of secondary metabolites. In the present chapter, we will discuss the importance of secondary metabolite production in plants, animals, and microbes. We will also discussthe machinery involved for increasing the production of secondary metabolites. ? 2018 Nova Science Publishers, Inc.Item Health promoting activity of myricetin: A natural flavonoid(Nova Science Publishers, Inc., 2017) Singh, P.; Bast, Felix; Kumar, S.; Kundu, P.Myricetin is a plant-derived flavonoid and is well known for its nutraceuticals value. It is a natural flavonol with hydroxyl groups at 3, 5, 7, 3', 4' and 5' positions, which found in fruits, vegetables, tea, berries and red wine. Furthermore, it can perform the functions including antiinflammation, anti-oxidative stress, anti-inflammatory, anti-apoptotic, neuroprotective properties, and anti-hyperlipidemia. All of those functions may contribute the prevention of cancer, diabetes, and neurodegenerative disease. In the present book chapter, a comprehensive discussion has been made to address the potential benefits of myricetin on cancer, diabetes, and neurodegenerative disease. We also discuss the underlined mechanism involved in the biological action of myricetin. ? 2017 Nova Science Publishers, Inc.Item Glycogenolysis(Nova Science Publishers, Inc., 2017) Kushwaha, P.P.; Kumar, S.In animals glycogen is the main storage form of fuel material. It is a multi-branched polysaccharide of glucose. Excess of glucose in body get stored in the form of glycogen for further utilization. In future when the body gets starvation condition the stored glycogen in the body gets split into its monomer constituent glucose. The glucose is then utilized by the body to fulfill the energy requirement. Breakdown of glycogen is known as glycogenolysis. It is a step by step process that produces glucose. In the present chapter we will discuss the structure and function of glycogen. The detailed mechanism of glycogenolysis is discussed in the chapter. Moreover we also discussed the regulation of this metabolic process. ? 2017 Nova Science Publishers, Inc. All rights reserved.