Development And Characterization Of SSR Markers In Calotropis Procera
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Date
2018
Authors
Journal Title
Journal ISSN
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Publisher
Central University of Punjab
Abstract
Calotropis procera is a weed growing around the arid and semi-arid areas of the
tropical and subtropical regions. The species has the potential to replace cotton
and resolve the high rates of cotton-based products and at the same time reduce
the use of synthetic non-biodegradable fibres. It has been used in various
medicinal formulations from a long time. There is a need for developing molecular
markers to facilitate breeding and genetic improvement of its varieties. Out of 12.6
million raw reads, 84636 reads were obtained after quality check and filtering.
93.7% of alignment rate and 76.31% of core genes were detected in Bowtie and
BUSCO assessment, respectively. An average of 5.5 SSRs/Mb was obtained in
72.349 Mb transcriptome. 25 SSR markers were selected for characterization on
30 individuals representing 3 populations. 13 SSR loci were found polymorphic. In
the population structure analysis a moderate level of genetic diversity (Na=3.9231,
He=0.633) was predicted. The genetic variation among the population was found
to be only 7% whereas within the population, it was 93%. The mean Shannon?s
information index obtained was 1.1, showing significant diversity richness in the
populations. The Dendrogram showed that the population from Bathinda and
Ambala are closely related to each other than those from Barnala. The gene flow
between Bathinda and Ambala population was found significantly high (6.439). The
present set of loci had a high PIC value of 0.506 that makes them very informative
to be further used for large scale studies.
Description
Keywords
Population structure, SSR markers, Calotropis procera, gene flow, cotton
Citation
Vandana (2018) Development And Characterization Of SSR
Markers In Calotropis Procera.