Development And Characterization Of SSR Markers In Calotropis Procera

dc.contributor.authorVandana
dc.contributor.supervisorBhardwaj, Pankaj
dc.date.accessioned2018-08-31T04:13:51Z
dc.date.accessioned2024-08-13T10:46:52Z
dc.date.available2018-08-31T04:13:51Z
dc.date.available2024-08-13T10:46:52Z
dc.date.issued2018
dc.description.abstractCalotropis procera is a weed growing around the arid and semi-arid areas of the tropical and subtropical regions. The species has the potential to replace cotton and resolve the high rates of cotton-based products and at the same time reduce the use of synthetic non-biodegradable fibres. It has been used in various medicinal formulations from a long time. There is a need for developing molecular markers to facilitate breeding and genetic improvement of its varieties. Out of 12.6 million raw reads, 84636 reads were obtained after quality check and filtering. 93.7% of alignment rate and 76.31% of core genes were detected in Bowtie and BUSCO assessment, respectively. An average of 5.5 SSRs/Mb was obtained in 72.349 Mb transcriptome. 25 SSR markers were selected for characterization on 30 individuals representing 3 populations. 13 SSR loci were found polymorphic. In the population structure analysis a moderate level of genetic diversity (Na=3.9231, He=0.633) was predicted. The genetic variation among the population was found to be only 7% whereas within the population, it was 93%. The mean Shannon?s information index obtained was 1.1, showing significant diversity richness in the populations. The Dendrogram showed that the population from Bathinda and Ambala are closely related to each other than those from Barnala. The gene flow between Bathinda and Ambala population was found significantly high (6.439). The present set of loci had a high PIC value of 0.506 that makes them very informative to be further used for large scale studies.en_US
dc.identifier.accessionnoT00769
dc.identifier.citationVandana (2018) Development And Characterization Of SSR Markers In Calotropis Procera.en_US
dc.identifier.urihttps://kr.cup.edu.in/handle/32116/1960
dc.language.isoen_USen_US
dc.publisherCentral University of Punjaben_US
dc.subjectPopulation structureen_US
dc.subjectSSR markersen_US
dc.subjectCalotropis proceraen_US
dc.subjectgene flowen_US
dc.subjectcottonen_US
dc.titleDevelopment And Characterization Of SSR Markers In Calotropis Proceraen_US
dc.typeMaster Dissertationen_US

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