| dc.description.abstract | Cancer is regarded as uncontrolled progression and spread of cells. Cancer is not
a singular, specific disease but a group of variable tissue responses that result in
uncontrolled cell growth. Healthy cells have a specific size, structure, function and
growth rate that best serves the needs of the tissues they compose. Cancer is one
of the leading of morbidity and mortality worldwide, with approximately 14 million
new cases in 2017. Breast cancer (BC) is a disease where cells in the tissue of the
breast cancer grow and divide without normal control.
Estrogen receptor is a group of proteins (or a twelve helix protein) present inside
the cells of the female reproductive tissues or located in the nucleus of cells. ER?,
ER? and ER gamma have different responses and they are located in different
tissues. Quinone forms of catechol estrogen binds to DNA and forms adduct. Semi
Quinone intermediates are free radicals can bind with oxygen to producing
superoxide radicals. Superoxide radicals can attack and alter the structure of DNA
and causing Breast cancer. Various synthetic drugs (Tamoxifen & Raloxifene) are
used for treatment of breast cancer, but numerous side effects like menopausal symptoms, vaginal dryness, low libido, mood swings and Nausea. The discovery of
novel natural drugs is important for reduction of side-effects, high selectivity, low
toxicity, and better killing of cancer cells. Phytoestrogens are one the best category
of natural products used for treatment of breast cancer. Phytoestrogens have
similar structure to the endogenous estrogen. Distance between the hydroxyl
groups is 14.5 A0 is similar to estrogen. Asparagus racemosus contain large
number of phytoestrogens. In this context, the aim of the present study was to
explore the roots of Asparagus racemosus in the terms of its medicinal values for
Breast cancer. Anticancer activity of different extracts were evaluated by
performing In vitro study by using Breast cancer cell lines T-47 D. from the
Preliminary phytochemical investigation of extracts demonstrated that methanolic
extract and Aqueous methanolic extract contain large number of phytoestrogens.
Aqueous methanolic extract and methanolic extract showed maximum IC50 value
as compare to other extract. Isolation of molecules from methanol extract, total four
molecules isolated from methanol extract and three molecules from aqueous
methanol extract. Moreover, in silico study of reported phytoestrogen from
Asparagus racemosus was also carried out using glide docking to investigate
interaction pattern with estrogen receptor ? and estrogen receptor ?. The top
docking score was obtained for Rutin (Estrogen receptor ?) and Quercetin
(Estrogen receptor ?). Tamoxifen and raloxifene used as standard for estrogen
receptor ? and oestradiol used as standard for estrogen receptor ?. From the
ADME study demonstrated that maximum flavonoids has highest oral absorption
as compare to other. The results showed that phytoestrogens are expected
prospective candidate for regulatory tumor progression with a special emphasis in
breast cancer progression. | en_US |
