| dc.contributor.author | Samal, Pallavi | |
| dc.date.accessioned | 2018-08-31T04:13:39Z | |
| dc.date.available | 2018-08-31T04:13:39Z | |
| dc.date.issued | 2018 | |
| dc.identifier.citation | Samal, Pallavi (2018) Role of Helicobacter pylori Enriched Media in Inducing Oxidative Stress in Human Cell lines. | en_US |
| dc.identifier.uri | http://kr.cup.edu.in/handle/32116/1828 | |
| dc.description.abstract | Helicobacter pylori is a gram-negative, helical, microaerophilic bacterium which colonizes the human gastrointestinal tract. Vacuolating cytotoxin A (VacA) is one of the major virulent factors. Reactive oxygen species (ROS) and Reactive nitrogen species (RNS) produced by the immune and epithelial cells damage the host cell thereby resulting in a persistent infection. The prolonged infection results in chronic inflammation, oxidative stress and DNA damage. The microbe affects the major macromolecules of the host tissues lipids, proteins and DNA which leads to lipid peroxidation, protein oxidation and DNA fragmentation hence making the oxidative stress a deleterious damage. Role of H. pylori enriched media (HPEM) in inducing oxidative stress in two human cell lines AGS (human gastric cell line) and THP-1(human monocytic cell line) was studied in present work. The AGS cells and THP-1 cells was treated with various concentrations of HPEM and oxidative stress was evaluated by examining the levels of protein carbonyls, TBARS (thiobarbituric acid reactive species) and nitric oxide by spectophotometric and Western blotting methods. The oxidative stress induced by HPEM showed damaging effects on the cell membrane, protein and produced significantly high nitric oxide (NO) when compared with the untreated controls. From the present work it can be concluded that HPEM exposure to THP-1 and AGS cells enhanced the oxidative stress which leads to cellular damage and is ultimately responsible for the severe H. pylori associated fatal complications during its pathogenesis. | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | Central University of Punjab | en_US |
| dc.subject | H pylori | en_US |
| dc.subject | VacA | en_US |
| dc.subject | ROS | en_US |
| dc.subject | RNS | en_US |
| dc.subject | HPEM | en_US |
| dc.subject | THP-1 | en_US |
| dc.subject | AGS | en_US |
| dc.subject | Oxidative stress | en_US |
| dc.title | Role of Helicobacter pylori Enriched Media in Inducing Oxidative Stress in Human Cell lines | en_US |
| dc.type | Master Dissertation | en_US |
| dc.contributor.supervisor | Dhiman, Monisha | |
| dc.identifier.accessionno | T00662 | |
