Identification Of Ganoderic Acids And Derivatives From Fruiting Bodies Of Ganoderma Lucidum
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Ganoderic acids and other bioactive triterpene metabolites from crude extract of wood decaying fungus Ganoderma lucidum from two different hosts Acacia and Azadirachta were to be isolated as proposed. The method for extraction was simple and divided into three parts, 1. Solvent selection, 2. Dissolving metabolites into solvent for maximization of number of detected metabolites, 3. Extraction of metabolites from solvent. A variety of methods for maximizing dissolution in solvent based on pharmaceutical extraction protocols were employed. Sonication was chosen as the best method with detection of 5 distinct spots (metabolites) from TLC. Ethanol based sample extracts were packed inside silica gel column while using chloroform as the mobile phases in column chromatography. Some metabolites were isolated and confirmed by TLC. Due to time bound project work the further isolation was paused and the crude extracts were forwarded for GC-MS detection and analysis of constituent metabolites. After preparation of sample and analysis in gas chromatography mass spectroscopy (GC-MS), 16 metabolites were found in chloroform extract of ganoderma having azadirachta as host and 24 metabolites were found from acacia host associated fungal crude extract. The detected metabolites were alkaloids, phenols, fatty acids (and precursors), esters, di-ene compounds and sterols (terpenes).Terpenes (C28-Phytosterols) were found with molecular weight 396-398. 7,22-Ergostadienol with area 2.91% and 1.19% is found in Acacia and Azadirachta samples. Moreover another metabolite 7,22- Ergostadienone with area 0.61% is also found in Acacia (Host) sample this metabolite has various bio-active properties like anti-inflamatory and cytotoxic properties. These are derivatives of the ergosterols found in fungal body. These structures are products of lanosterol, (squalene derived products) they have many anthropocentric exploitive uses and they resemble the metabolite backbone (derivatives) we were initially looking for.
- Master's 
- Plant Sciences-Master Dissertation