| dc.contributor.author | Chambiyal, Gourav | |
| dc.date.accessioned | 2018-08-31T04:13:39Z | |
| dc.date.available | 2018-08-31T04:13:39Z | |
| dc.date.issued | 2018 | |
| dc.identifier.citation | Chambiyal,Gourav (2018) Antibacterial and Anticancer property of bioactive secondary metabolites from Actinomycetes isolated from different regions of Himachal Pradesh | en_US |
| dc.identifier.uri | http://kr.cup.edu.in/handle/32116/1830 | |
| dc.description.abstract | The prime objective of the present work is to isolate, characterize and purify soil
microbes and to know about their antibacterial and anticancer properties. Soil
samples were obtained from different locations in Dharamsala, Himachal Pradesh,
India. Serial dilutions of the samples were made and plated on starch casein agar.
Screening, purification and further tests were performed on the isolates to find the
potential colonies. Biochemical and morphological characterization was done as
well. Following characterization, isolates were used to extract bioactive secondary
metabolites which can be anti-bacterial and anti-cancer. Secondary screening for
antibacterial properties of isolates was evaluated by Agar well diffusion method.
Further, the isolates were subjected to the GC-MS and MTT cytotoxic assay. Totally 7
isolates of actinomycetes were isolated and out of them only 4 were tested for
antagonistic activity against 4 pathogenic microorganisms. Isolates M1,and M3 of
dilution 10-1 were active, while M2 and M4 showed less activity against the pathogenic
microorganisms. All actinomycetes isolates showed antibacterial activity against S.
enterica and P. putida while they showed less activity against S. aureus and E.coli.
These isolates showed antibacterial and anti-cancer activities and may be used for
the growth of new antibiotics for pharmaceutical or agricultural purposes. | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | Central University of Punjab | en_US |
| dc.subject | Agar well diffusion method | en_US |
| dc.subject | antimicrobial activity | en_US |
| dc.subject | secondary screening | en_US |
| dc.subject | pathogenic microorganisms | en_US |
| dc.subject | antagonistic | en_US |
| dc.subject | bioactive secondary metabolites | en_US |
| dc.subject | starch casein agar | en_US |
| dc.title | Antibacterial and Anticancer property of bioactive secondary metabolites from Actinomycetes isolated from different regions of Himachal Pradesh | en_US |
| dc.type | Master Dissertation | en_US |
| dc.contributor.supervisor | Kushawaha Pramod K. | |
| dc.identifier.accessionno | T00667 | |
